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Evaluation of a real-time method of simultaneous amplification and testing in diagnosis of Mycoplasma pneumoniae infection in children with pneumonia
Mycoplasma pneumoniae (M. pneumoniae) infection can cause community acquired pneumonia in children. A real-time method of simultaneous amplification and testing of M. pneumoniae (SAT-MP) was developed to diagnose M. pneumoniae targeting a region of the ribosomal RNA. The SAT-MP assay can accurately...
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| Published in: | PloS one 2017-05, Vol.12 (5), p.e0177842-e0177842 |
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| creator | Li, Wei Fang, You-Hong Shen, Hong-Qiang Yang, De-Hua Shu, Qiang Shang, Shi-Qiang |
| description | Mycoplasma pneumoniae (M. pneumoniae) infection can cause community acquired pneumonia in children. A real-time method of simultaneous amplification and testing of M. pneumoniae (SAT-MP) was developed to diagnose M. pneumoniae targeting a region of the ribosomal RNA. The SAT-MP assay can accurately identify M. pneumoniae with a detection range from 101 to 107 CFU/ml. In this study, the specimens from 315 children with pneumonia were collected and analyzed by SAT-MP in parallel with real-time PCR method and IgM ELISA assay. The positive rates of these specimens examined by SAT-MP assay, real-time PCR method and IgM ELISA assay were 16.51%, 15.56% and 12.70% respectively. While there was statistical significance (p = 0.04) between SAT-MP assay and IgM ELISA assay, no statistical significance (p = 0.25) was found between SAT-MP assay and real-time PCR method and these two methods had high consistency (kappa value = 0.97). These findings indicate that the newly developed SAT-MP assay is a rapid, sensitive and specific method for identifying M. pneumoniae with potential clinical application in the early diagnosis of M. pneumoniae infection. |
| doi_str_mv | 10.1371/journal.pone.0177842 |
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A real-time method of simultaneous amplification and testing of M. pneumoniae (SAT-MP) was developed to diagnose M. pneumoniae targeting a region of the ribosomal RNA. The SAT-MP assay can accurately identify M. pneumoniae with a detection range from 101 to 107 CFU/ml. In this study, the specimens from 315 children with pneumonia were collected and analyzed by SAT-MP in parallel with real-time PCR method and IgM ELISA assay. The positive rates of these specimens examined by SAT-MP assay, real-time PCR method and IgM ELISA assay were 16.51%, 15.56% and 12.70% respectively. While there was statistical significance (p = 0.04) between SAT-MP assay and IgM ELISA assay, no statistical significance (p = 0.25) was found between SAT-MP assay and real-time PCR method and these two methods had high consistency (kappa value = 0.97). These findings indicate that the newly developed SAT-MP assay is a rapid, sensitive and specific method for identifying M. pneumoniae with potential clinical application in the early diagnosis of M. pneumoniae infection.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0177842</identifier><identifier>PMID: 28520818</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Absorbance ; Age ; Amplification ; Antibodies ; Assaying ; Bacteria ; Beads ; Biology and Life Sciences ; Biotechnology ; Buffers ; Care and treatment ; Cell culture ; Centrifuging ; Child ; Child, Preschool ; Children ; Clinical medicine ; Colony-forming cells ; Computer programs ; Deoxyribonucleic acid ; Diagnosis ; Dilution ; DNA ; DNA probes ; DNA-directed RNA polymerase ; Enzyme-linked immunosorbent assay ; Evaluation ; Extraction ; Female ; Fluorescence ; Fluorescent indicators ; Health aspects ; Hepatitis ; Hepatitis C ; Humans ; Immunoglobulin M ; Infant ; Infections ; Influence ; Laboratories ; Leukemia ; Lymphocytes T ; Lysis ; Male ; Medical diagnosis ; Medicine ; Medicine and Health Sciences ; Methods ; Molecular Diagnostic Techniques - methods ; Mycoplasma pneumoniae ; Mycoplasma pneumoniae - genetics ; Mycoplasma pneumoniae - immunology ; Pathogens ; Pediatrics ; Pellets ; Pneumonia ; Pneumonia, Mycoplasma - blood ; Pneumonia, Mycoplasma - microbiology ; Political activity ; Political aspects ; Polymerase chain reaction ; Populations ; Probes ; Public health ; Real-Time Polymerase Chain Reaction - methods ; Reproducibility of Results ; Research and Analysis Methods ; Respiratory tract ; Ribonuclease ; Ribonucleic acid ; RNA ; RNA polymerase ; Samples ; Sensitivity and Specificity ; Serologic Tests - methods ; Serological tests ; Statistical analysis ; Thorax ; Tuberculosis ; Viruses</subject><ispartof>PloS one, 2017-05, Vol.12 (5), p.e0177842-e0177842</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Li et al 2017 Li et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-2f79f959242510624cc9fc2cb10a171e6828ee1696138458962d73aca62268263</citedby><cites>FETCH-LOGICAL-c692t-2f79f959242510624cc9fc2cb10a171e6828ee1696138458962d73aca62268263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1899376509/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1899376509?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28520818$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Balish, Mitchell F.</contributor><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Fang, You-Hong</creatorcontrib><creatorcontrib>Shen, Hong-Qiang</creatorcontrib><creatorcontrib>Yang, De-Hua</creatorcontrib><creatorcontrib>Shu, Qiang</creatorcontrib><creatorcontrib>Shang, Shi-Qiang</creatorcontrib><title>Evaluation of a real-time method of simultaneous amplification and testing in diagnosis of Mycoplasma pneumoniae infection in children with pneumonia</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Mycoplasma pneumoniae (M. pneumoniae) infection can cause community acquired pneumonia in children. A real-time method of simultaneous amplification and testing of M. pneumoniae (SAT-MP) was developed to diagnose M. pneumoniae targeting a region of the ribosomal RNA. The SAT-MP assay can accurately identify M. pneumoniae with a detection range from 101 to 107 CFU/ml. In this study, the specimens from 315 children with pneumonia were collected and analyzed by SAT-MP in parallel with real-time PCR method and IgM ELISA assay. The positive rates of these specimens examined by SAT-MP assay, real-time PCR method and IgM ELISA assay were 16.51%, 15.56% and 12.70% respectively. While there was statistical significance (p = 0.04) between SAT-MP assay and IgM ELISA assay, no statistical significance (p = 0.25) was found between SAT-MP assay and real-time PCR method and these two methods had high consistency (kappa value = 0.97). These findings indicate that the newly developed SAT-MP assay is a rapid, sensitive and specific method for identifying M. pneumoniae with potential clinical application in the early diagnosis of M. pneumoniae infection.</description><subject>Absorbance</subject><subject>Age</subject><subject>Amplification</subject><subject>Antibodies</subject><subject>Assaying</subject><subject>Bacteria</subject><subject>Beads</subject><subject>Biology and Life Sciences</subject><subject>Biotechnology</subject><subject>Buffers</subject><subject>Care and treatment</subject><subject>Cell culture</subject><subject>Centrifuging</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Children</subject><subject>Clinical medicine</subject><subject>Colony-forming cells</subject><subject>Computer programs</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnosis</subject><subject>Dilution</subject><subject>DNA</subject><subject>DNA probes</subject><subject>DNA-directed RNA polymerase</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Evaluation</subject><subject>Extraction</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Fluorescent indicators</subject><subject>Health aspects</subject><subject>Hepatitis</subject><subject>Hepatitis C</subject><subject>Humans</subject><subject>Immunoglobulin M</subject><subject>Infant</subject><subject>Infections</subject><subject>Influence</subject><subject>Laboratories</subject><subject>Leukemia</subject><subject>Lymphocytes T</subject><subject>Lysis</subject><subject>Male</subject><subject>Medical diagnosis</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Methods</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Mycoplasma pneumoniae</subject><subject>Mycoplasma pneumoniae - genetics</subject><subject>Mycoplasma pneumoniae - immunology</subject><subject>Pathogens</subject><subject>Pediatrics</subject><subject>Pellets</subject><subject>Pneumonia</subject><subject>Pneumonia, Mycoplasma - blood</subject><subject>Pneumonia, Mycoplasma - microbiology</subject><subject>Political activity</subject><subject>Political aspects</subject><subject>Polymerase chain reaction</subject><subject>Populations</subject><subject>Probes</subject><subject>Public health</subject><subject>Real-Time Polymerase Chain Reaction - methods</subject><subject>Reproducibility of Results</subject><subject>Research and Analysis Methods</subject><subject>Respiratory tract</subject><subject>Ribonuclease</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA polymerase</subject><subject>Samples</subject><subject>Sensitivity and Specificity</subject><subject>Serologic Tests - methods</subject><subject>Serological tests</subject><subject>Statistical analysis</subject><subject>Thorax</subject><subject>Tuberculosis</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11rFDEUhgdRbK3-A9EBQfRi13zMZJIboZSqhUrBr9twNnNmN0sm2U4y1f4Q_6_Z7rbuSi8kFwknz_uGnI-ieE7JlPKGvluGcfDgpqvgcUpo08iKPSgOqeJsIhjhD3fOB8WTGJeE1FwK8bg4YLJmRFJ5WPw-vQI3QrLBl6EroRwQ3CTZHsse0yK062i0_egSeAxjLKFfOdtZs9GAb8uEMVk_L60vWwtzH6KNa9nnaxNWDmIP5crj2AdvATPVobnRZt4srGsH9OVPmxZ_qafFow5cxGfb_aj4_uH028mnyfnFx7OT4_OJEYqlCesa1alasYrVlAhWGaM6w8yMEqANRSGZRKRCCcplVUslWNtwMCAYy3eCHxUvN74rF6LeZjRqKpXijaiJysTZhmgDLPVqsD0M1zqA1TeBMMw1DMkah7quZGdQ1rMGaNVWs5liNSiCUFccBZrs9X772jjrsTXo0wBuz3T_xtuFnoer7Mx50zTZ4M3WYAiXY8667m006NymNJoqQmQuca7zUfHqH_T-322pOeQP5MqE_K5Zm-rjSlHJuGiqTE3vofJqsbcmt19nc3xP8HZPkJmEv9Icxhj12dcv_89e_NhnX--wi9ypaRGDG9fdFPfBagOaIcQ4YHeXZEr0enpus6HX06O305NlL3YLdCe6HRf-B4AIFtY</recordid><startdate>20170516</startdate><enddate>20170516</enddate><creator>Li, 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of a real-time method of simultaneous amplification and testing in diagnosis of Mycoplasma pneumoniae infection in children with pneumonia</title><author>Li, Wei ; Fang, You-Hong ; Shen, Hong-Qiang ; Yang, De-Hua ; Shu, Qiang ; Shang, Shi-Qiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-2f79f959242510624cc9fc2cb10a171e6828ee1696138458962d73aca62268263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Absorbance</topic><topic>Age</topic><topic>Amplification</topic><topic>Antibodies</topic><topic>Assaying</topic><topic>Bacteria</topic><topic>Beads</topic><topic>Biology and Life Sciences</topic><topic>Biotechnology</topic><topic>Buffers</topic><topic>Care and treatment</topic><topic>Cell culture</topic><topic>Centrifuging</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Children</topic><topic>Clinical medicine</topic><topic>Colony-forming cells</topic><topic>Computer programs</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnosis</topic><topic>Dilution</topic><topic>DNA</topic><topic>DNA probes</topic><topic>DNA-directed RNA polymerase</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Evaluation</topic><topic>Extraction</topic><topic>Female</topic><topic>Fluorescence</topic><topic>Fluorescent indicators</topic><topic>Health aspects</topic><topic>Hepatitis</topic><topic>Hepatitis C</topic><topic>Humans</topic><topic>Immunoglobulin M</topic><topic>Infant</topic><topic>Infections</topic><topic>Influence</topic><topic>Laboratories</topic><topic>Leukemia</topic><topic>Lymphocytes T</topic><topic>Lysis</topic><topic>Male</topic><topic>Medical diagnosis</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Methods</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>Mycoplasma pneumoniae</topic><topic>Mycoplasma pneumoniae - genetics</topic><topic>Mycoplasma pneumoniae - immunology</topic><topic>Pathogens</topic><topic>Pediatrics</topic><topic>Pellets</topic><topic>Pneumonia</topic><topic>Pneumonia, Mycoplasma - blood</topic><topic>Pneumonia, Mycoplasma - microbiology</topic><topic>Political activity</topic><topic>Political aspects</topic><topic>Polymerase chain reaction</topic><topic>Populations</topic><topic>Probes</topic><topic>Public health</topic><topic>Real-Time Polymerase Chain Reaction - methods</topic><topic>Reproducibility of Results</topic><topic>Research and Analysis Methods</topic><topic>Respiratory tract</topic><topic>Ribonuclease</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA polymerase</topic><topic>Samples</topic><topic>Sensitivity and Specificity</topic><topic>Serologic Tests - methods</topic><topic>Serological tests</topic><topic>Statistical 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Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Wei</au><au>Fang, You-Hong</au><au>Shen, Hong-Qiang</au><au>Yang, De-Hua</au><au>Shu, Qiang</au><au>Shang, Shi-Qiang</au><au>Balish, Mitchell F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of a real-time method of simultaneous amplification and testing in diagnosis of Mycoplasma pneumoniae infection in children with pneumonia</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-05-16</date><risdate>2017</risdate><volume>12</volume><issue>5</issue><spage>e0177842</spage><epage>e0177842</epage><pages>e0177842-e0177842</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Mycoplasma pneumoniae (M. pneumoniae) infection can cause community acquired pneumonia in children. A real-time method of simultaneous amplification and testing of M. pneumoniae (SAT-MP) was developed to diagnose M. pneumoniae targeting a region of the ribosomal RNA. The SAT-MP assay can accurately identify M. pneumoniae with a detection range from 101 to 107 CFU/ml. In this study, the specimens from 315 children with pneumonia were collected and analyzed by SAT-MP in parallel with real-time PCR method and IgM ELISA assay. The positive rates of these specimens examined by SAT-MP assay, real-time PCR method and IgM ELISA assay were 16.51%, 15.56% and 12.70% respectively. While there was statistical significance (p = 0.04) between SAT-MP assay and IgM ELISA assay, no statistical significance (p = 0.25) was found between SAT-MP assay and real-time PCR method and these two methods had high consistency (kappa value = 0.97). These findings indicate that the newly developed SAT-MP assay is a rapid, sensitive and specific method for identifying M. pneumoniae with potential clinical application in the early diagnosis of M. pneumoniae infection.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28520818</pmid><doi>10.1371/journal.pone.0177842</doi><tpages>e0177842</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-05, Vol.12 (5), p.e0177842-e0177842 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1899376509 |
| source | Open Access: PubMed Central; ProQuest Publicly Available Content database |
| subjects | Absorbance Age Amplification Antibodies Assaying Bacteria Beads Biology and Life Sciences Biotechnology Buffers Care and treatment Cell culture Centrifuging Child Child, Preschool Children Clinical medicine Colony-forming cells Computer programs Deoxyribonucleic acid Diagnosis Dilution DNA DNA probes DNA-directed RNA polymerase Enzyme-linked immunosorbent assay Evaluation Extraction Female Fluorescence Fluorescent indicators Health aspects Hepatitis Hepatitis C Humans Immunoglobulin M Infant Infections Influence Laboratories Leukemia Lymphocytes T Lysis Male Medical diagnosis Medicine Medicine and Health Sciences Methods Molecular Diagnostic Techniques - methods Mycoplasma pneumoniae Mycoplasma pneumoniae - genetics Mycoplasma pneumoniae - immunology Pathogens Pediatrics Pellets Pneumonia Pneumonia, Mycoplasma - blood Pneumonia, Mycoplasma - microbiology Political activity Political aspects Polymerase chain reaction Populations Probes Public health Real-Time Polymerase Chain Reaction - methods Reproducibility of Results Research and Analysis Methods Respiratory tract Ribonuclease Ribonucleic acid RNA RNA polymerase Samples Sensitivity and Specificity Serologic Tests - methods Serological tests Statistical analysis Thorax Tuberculosis Viruses |
| title | Evaluation of a real-time method of simultaneous amplification and testing in diagnosis of Mycoplasma pneumoniae infection in children with pneumonia |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T07%3A29%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20a%20real-time%20method%20of%20simultaneous%20amplification%20and%20testing%20in%20diagnosis%20of%20Mycoplasma%20pneumoniae%20infection%20in%20children%20with%20pneumonia&rft.jtitle=PloS%20one&rft.au=Li,%20Wei&rft.date=2017-05-16&rft.volume=12&rft.issue=5&rft.spage=e0177842&rft.epage=e0177842&rft.pages=e0177842-e0177842&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0177842&rft_dat=%3Cgale_plos_%3EA491823674%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c692t-2f79f959242510624cc9fc2cb10a171e6828ee1696138458962d73aca62268263%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1899376509&rft_id=info:pmid/28520818&rft_galeid=A491823674&rfr_iscdi=true |