Pathogenic seedborne viruses are rare but Phaseolus vulgaris endornaviruses are common in bean varieties grown in Nicaragua and Tanzania

• Published in: PloS one 2017-05, Vol.12 (5), p.e0178242-e0178242
• Main Authors: Nordenstedt, Noora, Marcenaro, Delfia, Chilagane, Daudi, Mwaipopo, Beatrice, Rajamäki, Minna-Liisa, Nchimbi-Msolla, Susan, Njau, Paul J R, Mbanzibwa, Deusdedith R, Valkonen, Jari P T
• Format: Article
• Language: English
• Subjects: Beans; Biology and Life Sciences; Breeding; Common beans; Comovirus - genetics; Continents; Cultivars; Disease transmission; Gene sequencing; Genomes; Grain; Greenhouses; Homology; Incidence; Insects; Legumes; New varieties; Nicaragua; Phaseolus - virology; Phaseolus vulgaris; Physiological aspects; Plant breeding; Plant parasites; Plant virus diseases; Plant viruses; Plant Viruses - genetics; Plant Viruses - physiology; Research and Analysis Methods; Ribonucleic acid; RNA; RNA, Viral - genetics; Seeds; Seeds - virology; Tanzania; Viruses
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0178242

Common bean (Phaseolus vulgaris) is an annual grain legume that was domesticated in Mesoamerica (Central America) and the Andes. It is currently grown widely also on other continents including Africa. We surveyed seedborne viruses in new common bean varieties introduced to Nicaragua (Central America) and in landraces and improved varieties grown in Tanzania (eastern Africa). Bean seeds, harvested from Nicaragua and Tanzania, were grown in insect-controlled greenhouse or screenhouse, respectively, to obtain leaf material for virus testing. Equal amounts of total RNA from different samples were pooled (30-36 samples per pool), and small RNAs were deep-sequenced (Illumina). Assembly of the reads (21-24 nt) to contiguous sequences and searches for homologous viral sequences in databases revealed Phaseolus vulgaris endornavirus 1 (PvEV-1) and PvEV-2 in the bean varieties in Nicaragua and Tanzania. These viruses are not known to cause symptoms in common bean and are considered non-pathogenic. The small-RNA reads from each pool of samples were mapped to the previously characterized complete PvEV-1 and PvEV-2 sequences (genome lengths ca. 14 kb and 15 kb, respectively). Coverage of the viral genomes was 87.9-99.9%, depending on the pool. Coverage per nucleotide ranged from 5 to 471, confirming virus identification. PvEV-1 and PvEV-2 are known to occur in Phaseolus spp. in Central America, but there is little previous information about their occurrence in Nicaragua, and no information about occurrence in Africa. Aside from Cowpea mild mosaic virus detected in bean plants grown from been seeds harvested from one region in Tanzania, no other pathogenic seedborne viruses were detected. The low incidence of infections caused by pathogenic viruses transmitted via bean seeds may be attributable to new, virus-resistant CB varieties released by breeding programs in Nicaragua and Tanzania.