Regulation of neural stem cell proliferation and differentiation by Kinesin family member 2a

In the developing neocortex, cells in the ventricular/subventricular zone are largely multipotent neural stem cells and neural progenitor cells. These cells undergo self-renewal at the early stage of embryonic development to amplify the progenitor pool and subsequently differentiate into neurons. It...

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Bibliographic Details
Published in:PloS one 2017-06, Vol.12 (6), p.e0179047-e0179047
Main Authors: Sun, Dong, Zhou, Xue, Yu, Hua-Li, He, Xiao-Xiao, Guo, Wei-Xiang, Xiong, Wen-Cheng, Zhu, Xiao-Juan
Format: Article
Language:English
Subjects:
Amplification
Animals
beta Catenin - genetics
Biology and Life Sciences
Brain
Cell Differentiation - genetics
Cell division
Cell growth
Cell proliferation
Cell Proliferation - genetics
Cell self-renewal
Cells (biology)
Cellular biology
Control
Developmental biology
Developmental stages
Differentiation
Education
Electroporation
Embryogenesis
Embryonic growth stage
Epigenetics
Female
Humans
Kinesin
Kinesin - genetics
Laboratory animals
Medicine and Health Sciences
Mice
Neocortex
Neocortex - growth & development
Neocortex - metabolism
Neural stem cells
Neural Stem Cells - metabolism
Neurogenesis
Neurogenesis - genetics
Neurology
Neurons
Neurons - metabolism
Neurosciences
Phosphorylation
Pregnancy
Proteins
Quantitative analysis
Repressor Proteins - genetics
Research and Analysis Methods
RNA, Small Interfering - genetics
Stem cells
Subventricular zone
Ventricle
β-Catenin
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Summary:In the developing neocortex, cells in the ventricular/subventricular zone are largely multipotent neural stem cells and neural progenitor cells. These cells undergo self-renewal at the early stage of embryonic development to amplify the progenitor pool and subsequently differentiate into neurons. It is thus of considerable interest to investigate mechanisms controlling the switch from neural stem cells or neural progenitor cells to neurons. Here, we present evidence that Kif2a, a member of the Kinesin-13 family, plays a role in regulating the proliferation and differentiation of neural stem cells or neural progenitor cells at embryonic day 13.5. Silencing Kif2a by use of in utero electroporation of Kif2a shRNA reduced neural stem cells proliferation or self-renewal but increased neuronal differentiation. We further found that knockdown of Kif2a decreased the protein level of β-catenin, which is a critical molecule for neocortical neurogenesis. Together, these results reveal an important function of Kif2a in embryonic neocortical neurogenesis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0179047