Intermittent hypoxia increases kidney tumor vascularization in a murine model of sleep apnea

We investigate the effects of intermittent hypoxia (IH), a characteristic feature of obstructive sleep apnea (OSA), on renal cancer progression in an animal and cell model. An in vivo mouse model (Balb/c, n = 50) of kidney cancer was used to assess the effect of IH on tumor growth, metastatic capaci...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2017-06, Vol.12 (6), p.e0179444-e0179444
Main Authors: Vilaseca, Antoni, Campillo, Noelia, Torres, Marta, Musquera, Mireia, Gozal, David, Montserrat, Josep M, Alcaraz, Antonio, Touijer, Karim A, Farré, Ramon, Almendros, Isaac
Format: Article
Language:English
Subjects:
Angiogenesis
Animal models
Animal models in research
Animals
Anoxia
Apnea
Biology and Life Sciences
Cancer
Carcinoma, Renal Cell - blood supply
Carcinoma, Renal Cell - complications
Carcinoma, Renal Cell - pathology
Care and treatment
Càncer de ronyó
Disease Models, Animal
Endothelial cells
Exposure
Gene expression
Genotype & phenotype
Hospitals
Hypoxia
Hypoxia - complications
Hypoxia - genetics
Hypoxia - pathology
Immune response
Immune system
In vitro methods and tests
Kidney
Kidney cancer
Kidney Neoplasms - blood supply
Kidney Neoplasms - complications
Kidney Neoplasms - genetics
Kidney Neoplasms - pathology
Macrophages
Macrophages - pathology
Male
Medicine and Health Sciences
Melanoma
Metastases
Metastasis
Mice, Inbred BALB C
Models animals en la investigació
Mortality
Neoplasm Metastasis
Neovascularization, Pathologic - genetics
Neovascularization, Pathologic - pathology
Obesity
Renal cancer
Renal cell carcinoma
Research and Analysis Methods
Rodents
Sleep
Sleep apnea
Sleep apnea syndromes
Sleep Apnea Syndromes - complications
Sleep Apnea Syndromes - genetics
Sleep Apnea Syndromes - pathology
Sleep disorders
Subcutaneous Tissue - pathology
Surgery
Síndromes d'apnea del son
Urology
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor A - metabolism
Vascularization
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c734t-5324def60c21712c54b0165e9d3a38afc5c0cedf92bbaf45edc32e11d111b91c3
cites cdi_FETCH-LOGICAL-c734t-5324def60c21712c54b0165e9d3a38afc5c0cedf92bbaf45edc32e11d111b91c3
container_end_page e0179444
container_issue 6
container_start_page e0179444
container_title PloS one
container_volume 12
creator Vilaseca, Antoni
Campillo, Noelia
Torres, Marta
Musquera, Mireia
Gozal, David
Montserrat, Josep M
Alcaraz, Antonio
Touijer, Karim A
Farré, Ramon
Almendros, Isaac
description We investigate the effects of intermittent hypoxia (IH), a characteristic feature of obstructive sleep apnea (OSA), on renal cancer progression in an animal and cell model. An in vivo mouse model (Balb/c, n = 50) of kidney cancer was used to assess the effect of IH on tumor growth, metastatic capacity, angiogenesis and tumor immune response. An in vitro model tested the effect of IH on RENCA cells, macrophages and endothelial cells. Tumor growth, metastatic capacity, circulating vascular endothelial growth factor (VEGF) and content of endothelial cells, tumor associated macrophages and their phenotype were assessed in the tumor. In vitro, VEGF cell expression was quantified.Although IH did not boost tumor growth, it significantly increased endothelial cells (p = 0.001) and circulating VEGF (p
doi_str_mv 10.1371/journal.pone.0179444
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1907553407</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A494844017</galeid><doaj_id>oai_doaj_org_article_47b93aafc8ce45f0905f881e1b6d2eb3</doaj_id><sourcerecordid>A494844017</sourcerecordid><originalsourceid>FETCH-LOGICAL-c734t-5324def60c21712c54b0165e9d3a38afc5c0cedf92bbaf45edc32e11d111b91c3</originalsourceid><addsrcrecordid>eNqNk12L1DAUhoso7rr6D0QLgujFjPlsmxthWfwYWFjw60oIaXo6k7VtapIuO_5605nuMJW9kFLaps_7npyTc5LkOUZLTHP87toOrlPNsrcdLBHOBWPsQXKKBSWLjCD68Oj9JHni_TVCnBZZ9jg5IQUXTBBxmvxcdQFca0KALqSbbW9vjUpNpx0oDz79ZaoOtmkYWuvSG-X10Chn_qhgbBexVKXt4EwHaWsraFJbp74B6FPVd6CeJo9q1Xh4Nj3Pku8fP3y7-Ly4vPq0uji_XOicsrDglLAK6gxpgnNMNGclwhkHUVFFC1VrrpGGqhakLFXNOFSaEsC4whiXAmt6lrzc-_aN9XIqjJdYoJxzylAeidWeqKy6lr0zrXJbaZWRuwXr1lK5YHQDkuWloCpGLTQwXiOBeF0UGHCZVQRKGr3eT9GGso17iYVzqpmZzv90ZiPX9kZylrE8Gw3w3kD7QUsHGpxWYSc8fIw3QTmRlOOiIFHzZgrq7O8BfJCt8RqaRnVgh12uBaOZKMZcX_2D3l-RiVqrmLTpahv3qkdTec4EKxiLPRWp5T1UvCpojY6dV5u4PhO8nQkiE-A2rNXgvVx9_fL_7NWPOfv6iN2AasLG22YY-9DPQTYV11nvHdSHg8FIjoNzVw05Do6cBifKXhwf6kF0Nyn0L1XRFGc</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1907553407</pqid></control><display><type>article</type><title>Intermittent hypoxia increases kidney tumor vascularization in a murine model of sleep apnea</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Vilaseca, Antoni ; Campillo, Noelia ; Torres, Marta ; Musquera, Mireia ; Gozal, David ; Montserrat, Josep M ; Alcaraz, Antonio ; Touijer, Karim A ; Farré, Ramon ; Almendros, Isaac</creator><contributor>Vinci, Maria Cristina</contributor><creatorcontrib>Vilaseca, Antoni ; Campillo, Noelia ; Torres, Marta ; Musquera, Mireia ; Gozal, David ; Montserrat, Josep M ; Alcaraz, Antonio ; Touijer, Karim A ; Farré, Ramon ; Almendros, Isaac ; Vinci, Maria Cristina</creatorcontrib><description>We investigate the effects of intermittent hypoxia (IH), a characteristic feature of obstructive sleep apnea (OSA), on renal cancer progression in an animal and cell model. An in vivo mouse model (Balb/c, n = 50) of kidney cancer was used to assess the effect of IH on tumor growth, metastatic capacity, angiogenesis and tumor immune response. An in vitro model tested the effect of IH on RENCA cells, macrophages and endothelial cells. Tumor growth, metastatic capacity, circulating vascular endothelial growth factor (VEGF) and content of endothelial cells, tumor associated macrophages and their phenotype were assessed in the tumor. In vitro, VEGF cell expression was quantified.Although IH did not boost tumor growth, it significantly increased endothelial cells (p = 0.001) and circulating VEGF (p&lt;0.001) in the in vivo model. Macrophages exposed to IH in vitro increased VEGF expression, whereas RENCA cells and endothelial cells did not. These findings are in keeping with previous clinical data suggesting that OSA has no effect on kidney cancer size and that the association observed between OSA and higher Fuhrman grade of renal cell carcinoma may be mediated though a proangiogenic process, with a key role of macrophages.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0179444</identifier><identifier>PMID: 28594929</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Angiogenesis ; Animal models ; Animal models in research ; Animals ; Anoxia ; Apnea ; Biology and Life Sciences ; Cancer ; Carcinoma, Renal Cell - blood supply ; Carcinoma, Renal Cell - complications ; Carcinoma, Renal Cell - pathology ; Care and treatment ; Càncer de ronyó ; Disease Models, Animal ; Endothelial cells ; Exposure ; Gene expression ; Genotype &amp; phenotype ; Hospitals ; Hypoxia ; Hypoxia - complications ; Hypoxia - genetics ; Hypoxia - pathology ; Immune response ; Immune system ; In vitro methods and tests ; Kidney ; Kidney cancer ; Kidney Neoplasms - blood supply ; Kidney Neoplasms - complications ; Kidney Neoplasms - genetics ; Kidney Neoplasms - pathology ; Macrophages ; Macrophages - pathology ; Male ; Medicine and Health Sciences ; Melanoma ; Metastases ; Metastasis ; Mice, Inbred BALB C ; Models animals en la investigació ; Mortality ; Neoplasm Metastasis ; Neovascularization, Pathologic - genetics ; Neovascularization, Pathologic - pathology ; Obesity ; Renal cancer ; Renal cell carcinoma ; Research and Analysis Methods ; Rodents ; Sleep ; Sleep apnea ; Sleep apnea syndromes ; Sleep Apnea Syndromes - complications ; Sleep Apnea Syndromes - genetics ; Sleep Apnea Syndromes - pathology ; Sleep disorders ; Subcutaneous Tissue - pathology ; Surgery ; Síndromes d'apnea del son ; Urology ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - genetics ; Vascular Endothelial Growth Factor A - metabolism ; Vascularization</subject><ispartof>PloS one, 2017-06, Vol.12 (6), p.e0179444-e0179444</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Vilaseca et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>cc-by (c) Vilaseca, Antoni et al., 2017 info:eu-repo/semantics/openAccess &lt;a href="http://creativecommons.org/licenses/by/3.0/es"&gt;http://creativecommons.org/licenses/by/3.0/es&lt;/a&gt;</rights><rights>2017 Vilaseca et al 2017 Vilaseca et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c734t-5324def60c21712c54b0165e9d3a38afc5c0cedf92bbaf45edc32e11d111b91c3</citedby><cites>FETCH-LOGICAL-c734t-5324def60c21712c54b0165e9d3a38afc5c0cedf92bbaf45edc32e11d111b91c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1907553407/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1907553407?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28594929$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Vinci, Maria Cristina</contributor><creatorcontrib>Vilaseca, Antoni</creatorcontrib><creatorcontrib>Campillo, Noelia</creatorcontrib><creatorcontrib>Torres, Marta</creatorcontrib><creatorcontrib>Musquera, Mireia</creatorcontrib><creatorcontrib>Gozal, David</creatorcontrib><creatorcontrib>Montserrat, Josep M</creatorcontrib><creatorcontrib>Alcaraz, Antonio</creatorcontrib><creatorcontrib>Touijer, Karim A</creatorcontrib><creatorcontrib>Farré, Ramon</creatorcontrib><creatorcontrib>Almendros, Isaac</creatorcontrib><title>Intermittent hypoxia increases kidney tumor vascularization in a murine model of sleep apnea</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>We investigate the effects of intermittent hypoxia (IH), a characteristic feature of obstructive sleep apnea (OSA), on renal cancer progression in an animal and cell model. An in vivo mouse model (Balb/c, n = 50) of kidney cancer was used to assess the effect of IH on tumor growth, metastatic capacity, angiogenesis and tumor immune response. An in vitro model tested the effect of IH on RENCA cells, macrophages and endothelial cells. Tumor growth, metastatic capacity, circulating vascular endothelial growth factor (VEGF) and content of endothelial cells, tumor associated macrophages and their phenotype were assessed in the tumor. In vitro, VEGF cell expression was quantified.Although IH did not boost tumor growth, it significantly increased endothelial cells (p = 0.001) and circulating VEGF (p&lt;0.001) in the in vivo model. Macrophages exposed to IH in vitro increased VEGF expression, whereas RENCA cells and endothelial cells did not. These findings are in keeping with previous clinical data suggesting that OSA has no effect on kidney cancer size and that the association observed between OSA and higher Fuhrman grade of renal cell carcinoma may be mediated though a proangiogenic process, with a key role of macrophages.</description><subject>Angiogenesis</subject><subject>Animal models</subject><subject>Animal models in research</subject><subject>Animals</subject><subject>Anoxia</subject><subject>Apnea</subject><subject>Biology and Life Sciences</subject><subject>Cancer</subject><subject>Carcinoma, Renal Cell - blood supply</subject><subject>Carcinoma, Renal Cell - complications</subject><subject>Carcinoma, Renal Cell - pathology</subject><subject>Care and treatment</subject><subject>Càncer de ronyó</subject><subject>Disease Models, Animal</subject><subject>Endothelial cells</subject><subject>Exposure</subject><subject>Gene expression</subject><subject>Genotype &amp; phenotype</subject><subject>Hospitals</subject><subject>Hypoxia</subject><subject>Hypoxia - complications</subject><subject>Hypoxia - genetics</subject><subject>Hypoxia - pathology</subject><subject>Immune response</subject><subject>Immune system</subject><subject>In vitro methods and tests</subject><subject>Kidney</subject><subject>Kidney cancer</subject><subject>Kidney Neoplasms - blood supply</subject><subject>Kidney Neoplasms - complications</subject><subject>Kidney Neoplasms - genetics</subject><subject>Kidney Neoplasms - pathology</subject><subject>Macrophages</subject><subject>Macrophages - pathology</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Melanoma</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Mice, Inbred BALB C</subject><subject>Models animals en la investigació</subject><subject>Mortality</subject><subject>Neoplasm Metastasis</subject><subject>Neovascularization, Pathologic - genetics</subject><subject>Neovascularization, Pathologic - pathology</subject><subject>Obesity</subject><subject>Renal cancer</subject><subject>Renal cell carcinoma</subject><subject>Research and Analysis Methods</subject><subject>Rodents</subject><subject>Sleep</subject><subject>Sleep apnea</subject><subject>Sleep apnea syndromes</subject><subject>Sleep Apnea Syndromes - complications</subject><subject>Sleep Apnea Syndromes - genetics</subject><subject>Sleep Apnea Syndromes - pathology</subject><subject>Sleep disorders</subject><subject>Subcutaneous Tissue - pathology</subject><subject>Surgery</subject><subject>Síndromes d'apnea del son</subject><subject>Urology</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>Vascularization</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLgujFjPlsmxthWfwYWFjw60oIaXo6k7VtapIuO_5605nuMJW9kFLaps_7npyTc5LkOUZLTHP87toOrlPNsrcdLBHOBWPsQXKKBSWLjCD68Oj9JHni_TVCnBZZ9jg5IQUXTBBxmvxcdQFca0KALqSbbW9vjUpNpx0oDz79ZaoOtmkYWuvSG-X10Chn_qhgbBexVKXt4EwHaWsraFJbp74B6FPVd6CeJo9q1Xh4Nj3Pku8fP3y7-Ly4vPq0uji_XOicsrDglLAK6gxpgnNMNGclwhkHUVFFC1VrrpGGqhakLFXNOFSaEsC4whiXAmt6lrzc-_aN9XIqjJdYoJxzylAeidWeqKy6lr0zrXJbaZWRuwXr1lK5YHQDkuWloCpGLTQwXiOBeF0UGHCZVQRKGr3eT9GGso17iYVzqpmZzv90ZiPX9kZylrE8Gw3w3kD7QUsHGpxWYSc8fIw3QTmRlOOiIFHzZgrq7O8BfJCt8RqaRnVgh12uBaOZKMZcX_2D3l-RiVqrmLTpahv3qkdTec4EKxiLPRWp5T1UvCpojY6dV5u4PhO8nQkiE-A2rNXgvVx9_fL_7NWPOfv6iN2AasLG22YY-9DPQTYV11nvHdSHg8FIjoNzVw05Do6cBifKXhwf6kF0Nyn0L1XRFGc</recordid><startdate>20170608</startdate><enddate>20170608</enddate><creator>Vilaseca, Antoni</creator><creator>Campillo, Noelia</creator><creator>Torres, Marta</creator><creator>Musquera, Mireia</creator><creator>Gozal, David</creator><creator>Montserrat, Josep M</creator><creator>Alcaraz, Antonio</creator><creator>Touijer, Karim A</creator><creator>Farré, Ramon</creator><creator>Almendros, Isaac</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>XX2</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20170608</creationdate><title>Intermittent hypoxia increases kidney tumor vascularization in a murine model of sleep apnea</title><author>Vilaseca, Antoni ; Campillo, Noelia ; Torres, Marta ; Musquera, Mireia ; Gozal, David ; Montserrat, Josep M ; Alcaraz, Antonio ; Touijer, Karim A ; Farré, Ramon ; Almendros, Isaac</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c734t-5324def60c21712c54b0165e9d3a38afc5c0cedf92bbaf45edc32e11d111b91c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Angiogenesis</topic><topic>Animal models</topic><topic>Animal models in research</topic><topic>Animals</topic><topic>Anoxia</topic><topic>Apnea</topic><topic>Biology and Life Sciences</topic><topic>Cancer</topic><topic>Carcinoma, Renal Cell - blood supply</topic><topic>Carcinoma, Renal Cell - complications</topic><topic>Carcinoma, Renal Cell - pathology</topic><topic>Care and treatment</topic><topic>Càncer de ronyó</topic><topic>Disease Models, Animal</topic><topic>Endothelial cells</topic><topic>Exposure</topic><topic>Gene expression</topic><topic>Genotype &amp; phenotype</topic><topic>Hospitals</topic><topic>Hypoxia</topic><topic>Hypoxia - complications</topic><topic>Hypoxia - genetics</topic><topic>Hypoxia - pathology</topic><topic>Immune response</topic><topic>Immune system</topic><topic>In vitro methods and tests</topic><topic>Kidney</topic><topic>Kidney cancer</topic><topic>Kidney Neoplasms - blood supply</topic><topic>Kidney Neoplasms - complications</topic><topic>Kidney Neoplasms - genetics</topic><topic>Kidney Neoplasms - pathology</topic><topic>Macrophages</topic><topic>Macrophages - pathology</topic><topic>Male</topic><topic>Medicine and Health Sciences</topic><topic>Melanoma</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Mice, Inbred BALB C</topic><topic>Models animals en la investigació</topic><topic>Mortality</topic><topic>Neoplasm Metastasis</topic><topic>Neovascularization, Pathologic - genetics</topic><topic>Neovascularization, Pathologic - pathology</topic><topic>Obesity</topic><topic>Renal cancer</topic><topic>Renal cell carcinoma</topic><topic>Research and Analysis Methods</topic><topic>Rodents</topic><topic>Sleep</topic><topic>Sleep apnea</topic><topic>Sleep apnea syndromes</topic><topic>Sleep Apnea Syndromes - complications</topic><topic>Sleep Apnea Syndromes - genetics</topic><topic>Sleep Apnea Syndromes - pathology</topic><topic>Sleep disorders</topic><topic>Subcutaneous Tissue - pathology</topic><topic>Surgery</topic><topic>Síndromes d'apnea del son</topic><topic>Urology</topic><topic>Vascular endothelial growth factor</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>Vascularization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vilaseca, Antoni</creatorcontrib><creatorcontrib>Campillo, Noelia</creatorcontrib><creatorcontrib>Torres, Marta</creatorcontrib><creatorcontrib>Musquera, Mireia</creatorcontrib><creatorcontrib>Gozal, David</creatorcontrib><creatorcontrib>Montserrat, Josep M</creatorcontrib><creatorcontrib>Alcaraz, Antonio</creatorcontrib><creatorcontrib>Touijer, Karim A</creatorcontrib><creatorcontrib>Farré, Ramon</creatorcontrib><creatorcontrib>Almendros, Isaac</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale_Opposing Viewpoints In Context</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing &amp; Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>PHMC-Proquest健康医学期刊库</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>ProQuest Agricultural &amp; Environmental Science</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Recercat</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vilaseca, Antoni</au><au>Campillo, Noelia</au><au>Torres, Marta</au><au>Musquera, Mireia</au><au>Gozal, David</au><au>Montserrat, Josep M</au><au>Alcaraz, Antonio</au><au>Touijer, Karim A</au><au>Farré, Ramon</au><au>Almendros, Isaac</au><au>Vinci, Maria Cristina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intermittent hypoxia increases kidney tumor vascularization in a murine model of sleep apnea</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2017-06-08</date><risdate>2017</risdate><volume>12</volume><issue>6</issue><spage>e0179444</spage><epage>e0179444</epage><pages>e0179444-e0179444</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>We investigate the effects of intermittent hypoxia (IH), a characteristic feature of obstructive sleep apnea (OSA), on renal cancer progression in an animal and cell model. An in vivo mouse model (Balb/c, n = 50) of kidney cancer was used to assess the effect of IH on tumor growth, metastatic capacity, angiogenesis and tumor immune response. An in vitro model tested the effect of IH on RENCA cells, macrophages and endothelial cells. Tumor growth, metastatic capacity, circulating vascular endothelial growth factor (VEGF) and content of endothelial cells, tumor associated macrophages and their phenotype were assessed in the tumor. In vitro, VEGF cell expression was quantified.Although IH did not boost tumor growth, it significantly increased endothelial cells (p = 0.001) and circulating VEGF (p&lt;0.001) in the in vivo model. Macrophages exposed to IH in vitro increased VEGF expression, whereas RENCA cells and endothelial cells did not. These findings are in keeping with previous clinical data suggesting that OSA has no effect on kidney cancer size and that the association observed between OSA and higher Fuhrman grade of renal cell carcinoma may be mediated though a proangiogenic process, with a key role of macrophages.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28594929</pmid><doi>10.1371/journal.pone.0179444</doi><tpages>e0179444</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2017-06, Vol.12 (6), p.e0179444-e0179444
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1907553407
source Publicly Available Content Database; PubMed Central
subjects Angiogenesis
Animal models
Animal models in research
Animals
Anoxia
Apnea
Biology and Life Sciences
Cancer
Carcinoma, Renal Cell - blood supply
Carcinoma, Renal Cell - complications
Carcinoma, Renal Cell - pathology
Care and treatment
Càncer de ronyó
Disease Models, Animal
Endothelial cells
Exposure
Gene expression
Genotype & phenotype
Hospitals
Hypoxia
Hypoxia - complications
Hypoxia - genetics
Hypoxia - pathology
Immune response
Immune system
In vitro methods and tests
Kidney
Kidney cancer
Kidney Neoplasms - blood supply
Kidney Neoplasms - complications
Kidney Neoplasms - genetics
Kidney Neoplasms - pathology
Macrophages
Macrophages - pathology
Male
Medicine and Health Sciences
Melanoma
Metastases
Metastasis
Mice, Inbred BALB C
Models animals en la investigació
Mortality
Neoplasm Metastasis
Neovascularization, Pathologic - genetics
Neovascularization, Pathologic - pathology
Obesity
Renal cancer
Renal cell carcinoma
Research and Analysis Methods
Rodents
Sleep
Sleep apnea
Sleep apnea syndromes
Sleep Apnea Syndromes - complications
Sleep Apnea Syndromes - genetics
Sleep Apnea Syndromes - pathology
Sleep disorders
Subcutaneous Tissue - pathology
Surgery
Síndromes d'apnea del son
Urology
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor A - metabolism
Vascularization
title Intermittent hypoxia increases kidney tumor vascularization in a murine model of sleep apnea
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T02%3A42%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Intermittent%20hypoxia%20increases%20kidney%20tumor%20vascularization%20in%20a%20murine%20model%20of%20sleep%20apnea&rft.jtitle=PloS%20one&rft.au=Vilaseca,%20Antoni&rft.date=2017-06-08&rft.volume=12&rft.issue=6&rft.spage=e0179444&rft.epage=e0179444&rft.pages=e0179444-e0179444&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0179444&rft_dat=%3Cgale_plos_%3EA494844017%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c734t-5324def60c21712c54b0165e9d3a38afc5c0cedf92bbaf45edc32e11d111b91c3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1907553407&rft_id=info:pmid/28594929&rft_galeid=A494844017&rfr_iscdi=true