SbCOMT (Bmr12) is involved in the biosynthesis of tricin-lignin in sorghum

• Published in: PloS one 2017-06, Vol.12 (6), p.e0178160-e0178160
• Main Authors: Eudes, Aymerick, Dutta, Tanmoy, Deng, Kai, Jacquet, Nicolas, Sinha, Anagh, Benites, Veronica T, Baidoo, Edward E K, Richel, Aurore, Sattler, Scott E, Northen, Trent R, Singh, Seema, Simmons, Blake A, Loqué, Dominique
• Format: Article
• Language: English
• Subjects: Alcohols; Analysis; BASIC BIOLOGICAL SCIENCES; Biochemistry; Bioengineering; Biology; Biology and Life Sciences; Biomass; Biosynthesis; Biosynthetic Pathways; Caffeate O-methyltransferase; Cell walls; Cellulose - metabolism; Chains (polymeric); Chemistry; Chimie; Chromones - metabolism; Corn; Crops; Ecology, environment; Energy crops; Engineering; Ethanol; Exports; Flavonoids - biosynthesis; Flavonoids - chemistry; Flowers & plants; Genetic aspects; Genomics; Laboratories; Life Sciences; Lignin; Lignin - biosynthesis; Lignin - chemistry; Luteolin - metabolism; Metabolites; Methanol - chemistry; Methylation; Methyltransferase; Microbiology and Parasitology; Monomers; Physical Sciences; Physical, chemical, mathematical & earth Sciences; Physique, chimie, mathématiques & sciences de la terre; Plant biomass; Plant Proteins - metabolism; Polymerization; Polymers; Properties; R&D; Renewable energy; Research & development; Research and analysis methods; Sinapyl alcohol; Sorghum; Sorghum - metabolism; Sorghum bicolor; Sustainable development; Symbiosis
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0178160

Lignin in plant biomass represents a target for engineering strategies towards the development of a sustainable bioeconomy. In addition to the conventional lignin monomers, namely p-coumaryl, coniferyl and sinapyl alcohols, tricin has been shown to be part of the native lignin polymer in certain monocot species. Because tricin is considered to initiate the polymerization of lignin chains, elucidating its biosynthesis and mechanism of export to the cell wall constitute novel challenges for the engineering of bioenergy crops. Late steps of tricin biosynthesis require two methylation reactions involving the pathway intermediate selgin. It has recently been demonstrated in rice and maize that caffeate O-methyltransferase (COMT) involved in the synthesis syringyl (S) lignin units derived from sinapyl alcohol also participates in the synthesis of tricin in planta. In this work, we validate in sorghum (Sorghum bicolor L.) that the O-methyltransferase responsible for the production of S lignin units (SbCOMT / Bmr12) is also involved in the synthesis of lignin-linked tricin. In particular, we show that biomass from the sorghum bmr12 mutant contains lower level of tricin incorporated into lignin, and that SbCOMT can methylate the tricin precursors luteolin and selgin. Our genetic and biochemical data point toward a general mechanism whereby COMT is involved in the synthesis of both tricin and S lignin units.