Detection of human cytomegalovirus in glioblastoma among Taiwanese subjects

• Published in: PloS one 2017-06, Vol.12 (6), p.e0179366-e0179366
• Main Authors: Yang, Ching-Fen, Ho, Hsiang-Ling, Lin, Shih-Chieh, Hsu, Chih-Yi, Ho, Donald Ming-Tak
• Format: Article
• Language: English
• Subjects: Adult; Age; Aged; Assaying; Bevacizumab; Biology and life sciences; Brain cancer; Brain Neoplasms - pathology; Brain Neoplasms - virology; Cancer therapies; Chemotherapy; Child; Cytomegalovirus; Cytomegalovirus - isolation & purification; Dehydrogenase; Deoxyribonucleic acid; DNA; Female; Gender; Gene expression; Genes; Glioblastoma; Glioblastoma - pathology; Glioblastoma - virology; Glioblastomas; Hospitals; Humans; Immunohistochemistry; Isocitrate dehydrogenase; Labels; Laboratories; Male; Marking; Medical prognosis; Medicine; Medicine and Health Sciences; Methylguanine; Middle Aged; Monoclonal antibodies; Mutation; NMR; Nuclear magnetic resonance; Pathogenesis; Pathology; Patients; Pediatrics; Polymerase chain reaction; Prognosis; Radiation therapy; Research and Analysis Methods; Ribonucleic acid; RNA; Survival Analysis; Taiwan; Targeted cancer therapy; Transcription; Tumors; Viral infections
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0179366

The relationship between human cytomegalovirus (HCMV) and glioblastoma (GBM) has been debated for more than a decade. We investigated the presence of HCMV genes, RNA and protein in GBMs and their relationships with tumor progression. Results of quantitative PCR for HCMV UL73, nested PCR for HCMV UL144, in situ hybridization (ISH) for RNA transcript, and immunohistochemistry (IHC) for protein expression and their relationship to the prognosis of 116 patients with GBM were evaluated. Nine (7.8%) cases revealed a low concentration of HCMV UL73, and only 2 of the 9 (1.7%) cases showed consistent positivity on repeat PCR testing. HCMV UL144, ISH and IHC assays were all negative. The HCMV UL73 positive cases did not show significant difference in the clinicopathological characters including age, gender, Karnofsky performance status, extent of resection, bevacizumab treatment, isocitrate dehydrogenase 1 mutation, O6-methylguanine-DNA-methyltranferase status and Ki67 labeling index, and did not reveal prognostic significance. As only one HCMV gene was detected at low concentration in 7.8% of GBMs and there was no evidence of transcription, protein expression or prognostic impact, we cannot conclude a relationship between HCMV and GBM in Taiwanese patients.