A human genotyping trial to estimate the post-feeding time from mosquito blood meals

Mosquitoes occur almost worldwide, and females of some species feed on blood from humans and other animals to support ovum maturation. In warm and hot seasons, such as the summer in Japan, fed mosquitoes are often observed at crime scenes. The current study attempted to estimate the time that elapse...

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Bibliographic Details
Published in:PloS one 2017-06, Vol.12 (6), p.e0179319-e0179319
Main Authors: Hiroshige, Yuuji, Hara, Masaaki, Nagai, Atsushi, Hikitsuchi, Tomoyuki, Umeda, Mitsuo, Kawajiri, Yumi, Nakayama, Koji, Suzuki, Koichi, Takada, Aya, Ishii, Akira, Yamamoto, Toshimichi
Format: Article
Language:English
Subjects:
Abdomen
Aedes
Animal Nutritional Physiological Phenomena
Animals
Aquatic insects
Asian tiger mosquito
Bioethics
Biology and Life Sciences
Blood
Blood meals
Crime
Culex
Cytochrome
Deoxyribonucleic acid
Digestion
DNA
DNA - chemistry
DNA - genetics
DNA - isolation & purification
Enzymes
Estimation
Feeding
Female
Females
Forensic medicine
Forensic sciences
Genetic aspects
Genomics
Genotyping
Genotyping Techniques - methods
Humans
Identification
Laboratories
Legal medicine
Malaria
Male
Maturation
Meals
Medicine and Health Sciences
Mitochondrial DNA
Mosquitoes
Multiplexing
Physiological aspects
Polymerase chain reaction
Quality control
R&D
Reagent Kits, Diagnostic
Research & development
Research and Analysis Methods
Seasons
Short tandem repeats
Studies
Summer
Tropical diseases
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Summary:Mosquitoes occur almost worldwide, and females of some species feed on blood from humans and other animals to support ovum maturation. In warm and hot seasons, such as the summer in Japan, fed mosquitoes are often observed at crime scenes. The current study attempted to estimate the time that elapsed since feeding from the degree of human DNA digestion in mosquito blood meals and also to identify the individual human sources of the DNA using genotyping in two species of mosquito: Culex pipiens pallens and Aedes albopictus. After stereomicroscopic observation, the extracted DNA samples were quantified using a human DNA quantification and quality control kit and were genotyped for 15 short tandem repeats using a commercial multiplexing kit. It took about 3 days for the complete digestion of a blood meal, and genotyping was possible until 2 days post-feeding. The relative peak heights of the 15 STRs and DNA concentrations were useful for estimating the post-feeding time to approximately half a day between 0 and 2 days. Furthermore, the quantitative ratios derived from STR peak heights and the quality control kit (Q129/Q41, Q305/Q41, and Q305/Q129) were reasonably effective for estimating the approximate post-feeding time after 2-3 days. We suggest that this study may be very useful for estimating the time since a mosquito fed from blood meal DNA, although further refinements are necessary to estimate the times more accurately.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0179319