Allele specific CAPS marker development and characterization of chalcone synthase gene in Indian mulberry (Morus spp., family Moraceae)

Chalcone synthase (CHS) is an essential enzyme in the phenylpropanoid pathway that catalyzes the first step in flavonoid biosynthesis in plants under diverse environmental stress. We have used CHS as a candidate gene in mulberry and developed Single Nucleotide Polymorphism (SNP) based co-dominant Cl...

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Bibliographic Details
Published in:PloS one 2017-06, Vol.12 (6), p.e0179189-e0179189
Main Authors: Arora, Vivek, Ghosh, M K, Pal, Soumili, Gangopadhyay, Gaurab
Format: Article
Language:English
Subjects:
Acyltransferases - chemistry
Acyltransferases - genetics
Agricultural production
Airborne microorganisms
Alleles
Amino Acid Sequence
Amplification
Berries
Binding sites
Biology and Life Sciences
Biosynthesis
Chalcone synthase
Correlation analysis
Deoxyribonucleic acid
DNA
Docking
Environmental stress
Enzymes
Flavonoids
Fungi
Gene expression
Gene polymorphism
Genes
Genetic aspects
Genetic Markers - genetics
Genetically altered foods
Genomics
Genotypes
Homology
Hybridization
Modelling
Molecular Docking Simulation
Moraceae
Morus
Morus - enzymology
Morus - genetics
Morus - microbiology
Phenotype
Phenotyping
Physiological aspects
Plant biology
Plant Diseases - microbiology
Plant resistance
Plants (botany)
Polymorphism
Polymorphism, Single Nucleotide
Powdery mildew
Protein Conformation
Proteins
Recombinants
Research and Analysis Methods
Single nucleotide polymorphisms
Single-nucleotide polymorphism
Stresses
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Summary:Chalcone synthase (CHS) is an essential enzyme in the phenylpropanoid pathway that catalyzes the first step in flavonoid biosynthesis in plants under diverse environmental stress. We have used CHS as a candidate gene in mulberry and developed Single Nucleotide Polymorphism (SNP) based co-dominant Cleaved Amplified Polymorphic Sequence (CAPS) marker associated with the CHS locus. The segregation pattern of the marker was studied in an F1 population derived from a hybridization program between two mulberry genotypes showing polymorphism for the CHS locus. Differential CHS activity of the recombinants has been correlated with the segregation pattern of the marker. Homology modelling and docking studies are performed for both the identified CHS alleles and correlated with respective CHS activity. Phenotyping of Powdery Mildew infected F1 population indicated a probable association with the CAPS marker.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0179189