Whole genome analysis of selected human and animal rotaviruses identified in Uganda from 2012 to 2014 reveals complex genome reassortment events between human, bovine, caprine and porcine strains

Rotaviruses of species A (RVA) are a common cause of diarrhoea in children and the young of various other mammals and birds worldwide. To investigate possible interspecies transmission of RVAs, whole genomes of 18 human and 6 domestic animal RVA strains identified in Uganda between 2012 and 2014 wer...

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Published in:PloS one 2017-06, Vol.12 (6), p.e0178855-e0178855
Main Authors: Bwogi, Josephine, Jere, Khuzwayo C, Karamagi, Charles, Byarugaba, Denis K, Namuwulya, Prossy, Baliraine, Frederick N, Desselberger, Ulrich, Iturriza-Gomara, Miren
Format: Article
Language:English
Subjects:
Analysis
Animals
Backbone
Biology and Life Sciences
Birds
Cattle
Children
Circulation
Classification
Computer and Information Sciences
Diarrhea
Disease transmission
Domestic animals
Epidemiology
Gene Rearrangement
Genes
Genetics
Genome, Viral - genetics
Genomes
Genomics
Genotype
Goats - virology
Humans
Immunology
Infections
Infectious diseases
Mammals
Medicine and Health Sciences
People and Places
Phylogeny
Research and Analysis Methods
Rotavirus
Rotavirus - classification
Rotavirus - genetics
Rotavirus - isolation & purification
Rotavirus - physiology
Species Specificity
Swine - virology
Transcription
Uganda
Virology
Viruses
Zoonoses
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cited_by cdi_FETCH-LOGICAL-c758t-dee83ed3670d617bdef698dbbe8adcc9dccc7d88397a905c6e87be223a0b0da3
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container_title PloS one
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creator Bwogi, Josephine
Jere, Khuzwayo C
Karamagi, Charles
Byarugaba, Denis K
Namuwulya, Prossy
Baliraine, Frederick N
Desselberger, Ulrich
Iturriza-Gomara, Miren
description Rotaviruses of species A (RVA) are a common cause of diarrhoea in children and the young of various other mammals and birds worldwide. To investigate possible interspecies transmission of RVAs, whole genomes of 18 human and 6 domestic animal RVA strains identified in Uganda between 2012 and 2014 were sequenced using the Illumina HiSeq platform. The backbone of the human RVA strains had either a Wa- or a DS-1-like genetic constellation. One human strain was a Wa-like mono-reassortant containing a DS-1-like VP2 gene of possible animal origin. All eleven genes of one bovine RVA strain were closely related to those of human RVAs. One caprine strain had a mixed genotype backbone, suggesting that it emerged from multiple reassortment events involving different host species. The porcine RVA strains had mixed genotype backbones with possible multiple reassortant events with strains of human and bovine origin.Overall, whole genome characterisation of rotaviruses found in domestic animals in Uganda strongly suggested the presence of human-to animal RVA transmission, with concomitant circulation of multi-reassortant strains potentially derived from complex interspecies transmission events. However, whole genome data from the human RVA strains causing moderate and severe diarrhoea in under-fives in Uganda indicated that they were primarily transmitted from person-to-person.
doi_str_mv 10.1371/journal.pone.0178855
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subjects Analysis
Animals
Backbone
Biology and Life Sciences
Birds
Cattle
Children
Circulation
Classification
Computer and Information Sciences
Diarrhea
Disease transmission
Domestic animals
Epidemiology
Gene Rearrangement
Genes
Genetics
Genome, Viral - genetics
Genomes
Genomics
Genotype
Goats - virology
Humans
Immunology
Infections
Infectious diseases
Mammals
Medicine and Health Sciences
People and Places
Phylogeny
Research and Analysis Methods
Rotavirus
Rotavirus - classification
Rotavirus - genetics
Rotavirus - isolation & purification
Rotavirus - physiology
Species Specificity
Swine - virology
Transcription
Uganda
Virology
Viruses
Zoonoses
title Whole genome analysis of selected human and animal rotaviruses identified in Uganda from 2012 to 2014 reveals complex genome reassortment events between human, bovine, caprine and porcine strains
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T02%3A33%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Whole%20genome%20analysis%20of%20selected%20human%20and%20animal%20rotaviruses%20identified%20in%20Uganda%20from%202012%20to%202014%20reveals%20complex%20genome%20reassortment%20events%20between%20human,%20bovine,%20caprine%20and%20porcine%20strains&rft.jtitle=PloS%20one&rft.au=Bwogi,%20Josephine&rft.date=2017-06-22&rft.volume=12&rft.issue=6&rft.spage=e0178855&rft.epage=e0178855&rft.pages=e0178855-e0178855&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0178855&rft_dat=%3Cgale_plos_%3EA543295297%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c758t-dee83ed3670d617bdef698dbbe8adcc9dccc7d88397a905c6e87be223a0b0da3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1912556199&rft_id=info:pmid/28640820&rft_galeid=A543295297&rfr_iscdi=true