Characterization and analysis of long non-coding rna (lncRNA) in In Vitro- and Ex Vivo-derived cardiac progenitor cells

Recent advancements in cell-based therapies for the treatment of cardiovascular disease (CVD) show continuing promise for the use of transplanted stem and cardiac progenitor cells (CPCs) to promote cardiac restitution. However, a detailed understanding of the molecular mechanisms that control the de...

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Bibliographic Details
Published in:PloS one 2017-06, Vol.12 (6), p.e0180096-e0180096
Main Authors: Arnone, Baron, Chen, Jake Y, Qin, Gangjian
Format: Article
Language:English
Subjects:
Analysis
Animals
Bioinformatics
Biological activity
Biology and life sciences
Biomedical engineering
Cardiomyocytes
Cardiovascular diseases
Cell Differentiation
Cell Line
Cells (biology)
Differentiation
Engineering schools
Epigenetics
Gene expression
Genes
Genomics
Heart
Heart attacks
Heart diseases
Heart failure
Homeostasis
In vitro methods and tests
Medicine and Health Sciences
Mice
Molecular modelling
Myocytes, Cardiac - cytology
Nkx2.5 protein
Non-coding RNA
Penicillin
Populations
Proteins
Regulators
Research and Analysis Methods
Ribonucleic acid
RNA
RNA, Long Noncoding - genetics
Stem cell transplantation
Stem cells
Stem Cells - cytology
Stem Cells - metabolism
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Summary:Recent advancements in cell-based therapies for the treatment of cardiovascular disease (CVD) show continuing promise for the use of transplanted stem and cardiac progenitor cells (CPCs) to promote cardiac restitution. However, a detailed understanding of the molecular mechanisms that control the development of these cells remains incomplete and is critical for optimizing their use in such therapy. Long non-coding (lnc) RNA has recently emerged as a crucial class of regulatory molecules involved in directing a variety of critical biological processes including development, homeostasis and disease. As such, a rising body of evidence suggests that they also play key regulatory roles in CPC development, though many questions remain regarding the expression landscape and specific identity of lncRNA involved in this process. To address this, we performed whole transcriptome sequencing of two murine CPC populations-Nkx2-5 EmGFP reporter-sorted embryonic stem (ES) cell-derived and ex vivo, cardiosphere-derived-in an effort to characterize their lncRNA profiles and potentially identify novel CPC regulators. The resulting sequencing data revealed an enrichment in both CPC populations for a panel of previously-identified lncRNA genes associated with cardiac differentiation. Additionally, a total of 1,678 differentially expressed and as-of-yet unannotated, putative lncRNA genes were found to be enriched for in the two CPC populations relative to undifferentiated ES cells.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0180096