Genetic factors affecting EBV copy number in lymphoblastoid cell lines derived from the 1000 Genome Project samples

• Published in: PloS one 2017-06, Vol.12 (6), p.e0179446-e0179446
• Main Authors: Mandage, Rajendra, Telford, Marco, Rodríguez, Juan Antonio, Farré, Xavier, Layouni, Hafid, Marigorta, Urko M, Cundiff, Caitlin, Heredia-Genestar, Jose Maria, Navarro, Arcadi, Santpere, Gabriel
• Format: Article
• Language: English
• Subjects: Association analysis; Bioinformatics; Biology and Life Sciences; Biotechnology; Cancer; Cell adhesion & migration; Cell Line, Tumor; Cell lines; Computer Simulation; Copy number; Copy number variations; Diseases; DNA Copy Number Variations; DNA, Viral - genetics; Environmental factors; Epstein-Barr virus; Evolutionary biology; Gene expression; Gene mapping; Genes; Genetic aspects; Genetic factors; Genomes; Geography; Herpes simplex; Herpes viruses; Herpesvirus 4, Human - genetics; Human populations; Humans; Infections; Infectious diseases; Infectious mononucleosis; Inhibitors; Lymphoblastoid cell lines; Lymphocytes B; Lymphoma; Mapping; Medicine and health sciences; Mononucleosis; Multiple sclerosis; Pathogens; Polymerase chain reaction; Polymorphism, Single Nucleotide; Populations; Publishing; Real time; Replication; Studies; Transcription; Viral infections; Viruses
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0179446

Epstein-Barr virus (EBV), human herpes virus 4, has been classically associated with infectious mononucleosis, multiple sclerosis and several types of cancers. Many of these diseases show marked geographical differences in prevalence, which points to underlying genetic and/or environmental factors. Those factors may include a different susceptibility to EBV infection and viral copy number among human populations. Since EBV is commonly used to transform B-cells into lymphoblastoid cell lines (LCLs) we hypothesize that differences in EBV copy number among individual LCLs may reflect differential susceptibility to EBV infection. To test this hypothesis, we retrieved whole-genome sequenced EBV-mapping reads from 1,753 LCL samples derived from 19 populations worldwide that were sequenced within the context of the 1000 Genomes Project. An in silico methodology was developed to estimate the number of EBV copy number in LCLs and validated these estimations by real-time PCR. After experimentally confirming that EBV relative copy number remains stable over cell passages, we performed a genome wide association analysis (GWAS) to try detecting genetic variants of the host that may be associated with EBV copy number. Our GWAS has yielded several genomic regions suggestively associated with the number of EBV genomes per cell in LCLs, unraveling promising candidate genes such as CAND1, a known inhibitor of EBV replication. While this GWAS does not unequivocally establish the degree to which genetic makeup of individuals determine viral levels within their derived LCLs, for which a larger sample size will be needed, it potentially highlighted human genes affecting EBV-related processes, which constitute interesting candidates to follow up in the context of EBV related pathologies.