RpoS role in virulence and fitness in enteropathogenic Escherichia coli

• Published in: PloS one 2017-06, Vol.12 (6), p.e0180381-e0180381
• Main Authors: Mata, Gardênia Márcia Silva Campos, Ferreira, Gerson Moura, Spira, Beny
• Format: Article
• Language: English
• Subjects: Adhesins; Antibiotics; Bacteria; Bacterial Adhesion - physiology; Bacterial Proteins - genetics; Bacterial Proteins - physiology; Biology and Life Sciences; Cell culture; Competition; Continuous culture; Developing countries; Diarrhea; E coli; Epithelial cells; Escherichia coli; Escherichia coli - growth & development; Escherichia coli - pathogenicity; Escherichia coli - physiology; Fitness; Gene expression; Gene Knockdown Techniques; Genetic aspects; Genomics; Infants; Intimin; Laboratories; LDCs; Medicine and Health Sciences; Mutation; Oxidation resistance; Oxidative stress; Pathogens; Physiological aspects; Reproductive fitness; Research and Analysis Methods; RNA polymerase; Sigma factor; Sigma Factor - genetics; Sigma Factor - physiology; Signal transduction; Small intestine; Stationary phase; Strain; Strains (organisms); Stress response; Virulence; Virulence (Microbiology)
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0180381

Enteropathogenic Escherichia coli (EPEC) is a diarrheagenic pathogen that afflicts infants in developing countries. The most important virulence trait of EPEC is its ability to intimately adhere to cells in the small intestine, and to elicit diarrhea. The alternative sigma factor RpoS is involved in the virulence of several bacterial species. RpoS coordinates the general stress response and accumulates in cells under stress or in the stationary phase. RpoS levels differ across E. coli strains. High-RpoS strains are highly resistant to environmental stresses, but usually display low nutritional competence, while low-RpoS strains show the opposite phenotype. Here we investigated whether RpoS plays a role in the virulence and fitness of two different EPEC strains, E2348/69 and LRT9. A rpoS null mutation had a small positive effect on LRT9 adherence to epithelial cells, but the expression of the EPEC adhesins BfpA and intimin was not significantly affected by the mutation. E2348/69 adherence was not significantly affected by the rpoS mutation. The intrinsic level of RpoS was higher in LRT9 than in E2348/69 while the latter adhered more strongly and expressed higher levels of the adhesin BfpA than the former. Knockout of rpoS strongly impaired resistance to oxidative, osmotic and acid stress in both E2348/69 and LRT9. However, strain E2348/69 was significantly more sensitive to oxidative stress than LRT9. Finally, competition assays showed that the rpoS mutant of LRT9 displayed higher fitness under continuous culture than its isogenic wild-type strain, while E2348/69 outcompeted its rpoS mutant. In conclusion, RpoS plays mostly a positive role in EPEC biology and at least in the case of strain E2348/69 it is not constrained by the trade-off between vegetative growth and stress resistance.