Dorso-ventral skin characterization of the farmed fish gilthead seabream (Sparus aurata)

• Published in: PloS one 2017-06, Vol.12 (6), p.e0180438-e0180438
• Main Authors: Cordero, Héctor, Ceballos-Francisco, Diana, Cuesta, Alberto, Esteban, María Ángeles
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Antibiotics; Aquaculture; Biology; Biology and Life Sciences; Cell Cycle; Cell size; Cytometry; Electron microscopy; Epidermis; Fish; Fish farming; Fish farms; Fishes; Flow cytometry; Gene expression; Gene Expression Profiling; Genes; Genetic aspects; Healing; Histology; Homeostasis; Image analysis; Image processing; Immune system; Immunoglobulins; Integrity; Light microscopy; Medicine and Health Sciences; Microscopy, Electron, Scanning; Pathogens; Physiological aspects; Polymerase chain reaction; Probiotics; Regeneration; Research and Analysis Methods; Scanning electron microscopy; Sea Bream - anatomy & histology; Shellfish; Skin; Skin - anatomy & histology; Skin - cytology; Skin - metabolism; Skin - ultrastructure; Skin Physiological Phenomena; Transcription; Wound Healing; Wounds and Injuries - pathology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0180438

The skin is the first barrier of defence in fish, protecting against any external stressor and preserving the integrity and homeostasis of the fish body. The aim of this study was to characterise gilthead seabream skin by isolating cells and studying the cell cycle by flow cytometry, to study the skin histology by scanning electron microscopy and the transcription level of some immune-relevant genes by RT-PCR. Furthermore, the results obtained from samples taken from the dorsal and the ventral part of the specimens are compared. No differences were observed in the cell cycle of cells isolated from the dorsal and ventral zones of the skin or in the gene expression of the genes studied in both epidermal zones. However, the epidermis thickness of the ventral skin was higher than that of the dorsal skin, as demonstrated by image analysis using light microscopy. Besides, scanning electron microscopy pointed to a greater cell size and area of microridges in the apical part of the dorsal epidermal cells compared with ventral skin epidermal cells. This study represents a step forward in our knowledge of the skin structure of an important farmed teleost, gilthead seabream, one of the most commonly farmed fish worldwide. Furthermore, for functional characterization, experimental wounds were carried out comparing the wound healing rate between the dorsal and ventral regions of skin over the time. The results showed higher ratio of wound healing in the ventral region, whose wounds were closed after 15 days, compared to dorsal region of skin. Taking into account all together, this study represents a step forward in our knowledge of the skin structure and skin regeneration of an important farmed teleost, gilthead seabream, one of the most commonly farmed fish worldwide.