Carbohydrate-mediated responses during zygotic and early somatic embryogenesis in the endangered conifer, Araucaria angustifolia

Three zygotic developmental stages and two somatic Araucaria angustifolia cell lines with contrasting embryogenic potential were analyzed to identify the carbohydrate-mediated responses associated with embryo formation. Using a comparison between zygotic and somatic embryogenesis systems, the non-st...

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Bibliographic Details
Published in:PloS one 2017-07, Vol.12 (7), p.e0180051-e0180051
Main Authors: Navarro, Bruno V, Elbl, Paula, De Souza, Amanda P, Jardim, Vinicius, de Oliveira, Leandro F, Macedo, Amanda F, Dos Santos, André L W, Buckeridge, Marcos S, Floh, Eny I S
Format: Article
Language:English
Subjects:
Araucariaceae
Biological research
Biology
Biology and Life Sciences
Biotechnology
Carbohydrate Metabolism
Carbohydrates
Cell lines
Cell walls
Coniferophyta - genetics
Coniferophyta - growth & development
Coniferophyta - metabolism
Developmental stages
Ecology
Embryonic development
Embryonic growth stage
Embryos
Endangered & extinct species
Endangered Species
Gene expression
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genes, Plant
Kinases
Laboratories
Metabolism
Metabolites
Morphology
Network analysis
Physical Sciences
Physiological aspects
Physiology
Plant sciences
Proteins
Seeds
Seeds - genetics
Seeds - growth & development
Seeds - metabolism
Somatic embryogenesis
Starch
Sucrose
Sugar
Transcriptome
Trees
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Summary:Three zygotic developmental stages and two somatic Araucaria angustifolia cell lines with contrasting embryogenic potential were analyzed to identify the carbohydrate-mediated responses associated with embryo formation. Using a comparison between zygotic and somatic embryogenesis systems, the non-structural carbohydrate content, cell wall sugar composition and expression of genes involved in sugar sensing were analyzed, and a network analysis was used to identify coordinated features during embryogenesis. We observed that carbohydrate-mediated responses occur mainly during the early stages of zygotic embryo formation, and that during seed development there are coordinated changes that affect the development of the different structures (embryo and megagametophyte). Furthermore, sucrose and starch accumulation were associated with the responsiveness of the cell lines. This study sheds light on how carbohydrate metabolism is influenced during zygotic and somatic embryogenesis in the endangered conifer species, A. angustifolia.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0180051