Resveratrol ameliorates diet-induced dysregulation of lipid metabolism in zebrafish (Danio rerio)

• Published in: PloS one 2017-07, Vol.12 (7), p.e0180865-e0180865
• Main Authors: Ran, Gai, Ying, Li, Li, Lin, Yan, Qiaoqiao, Yi, Weijie, Ying, Chenjiang, Wu, Hongmei, Ye, Xiaolei
• Format: Article
• Language: English
• Subjects: AMP; AMP-activated protein kinase; Animals; Antioxidants - pharmacology; Biology and Life Sciences; Body length; Body weight; Caloric Restriction - adverse effects; Cardiovascular diseases; Caveolin; Caveolin 1 - genetics; Caveolin 1 - metabolism; Caveolin-1; Cell death; Chronic diseases; Condition factor; Cysts; Danio rerio; Diabetes mellitus; Diet; Diet - methods; Dosage and administration; Eating - physiology; Female; Fish; Gene Expression Regulation - drug effects; Health aspects; Health risks; Heart diseases; Homology; Kinases; Li Ying; Light levels; Lipid metabolism; Lipid Metabolism - drug effects; Lipid Metabolism - genetics; Male; Males; Medicine and Health Sciences; Metabolism; Microtubule-associated protein 1; Microtubule-Associated Proteins - genetics; Microtubule-Associated Proteins - metabolism; Nutrient deficiency; Obesity; Peroxisome proliferator-activated receptors; Phosphorylation; PPAR gamma - genetics; PPAR gamma - metabolism; Preventive medicine; Protein Kinases - genetics; Protein Kinases - metabolism; Research and Analysis Methods; Resveratrol; Risk factors; Rodents; Signal Transduction; SIRT1 protein; Sirtuin 1 - genetics; Sirtuin 1 - metabolism; Stilbenes - pharmacology; Transcription Factors - genetics; Transcription Factors - metabolism; Triglycerides - metabolism; Weight reduction; Zebrafish; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0180865

Defective lipid metabolism is associated with increased risk of various chronic diseases, such as obesity, cardiovascular diseases, and diabetes. Resveratrol (RSV), a natural polyphenol, has been shown the potential of ameliorating disregulations of lipid metabolism. The objective of this study was to investigate the effects of feed intake and RSV on lipid metabolism in zebrafish (Danio rerio). The adult males were randomly allocated to 6 groups: control (Con, 8 mg cysts/fish/day), control with 20 μmol/L RSV (Con+RSV), calorie restriction (CR, 5 mg cysts/fish/day), calorie restriction with RSV (CR+RSV), overfeed (OF, 60 mg cysts/fish/day), and overfeed with RSV (OF+RSV) groups. The treatment period was 8 weeks. Results showed that CR reduced body length, body weight, and condition factor of zebrafish. CR reduced levels of plasma triglyceride (TG) and induced protein expression of phosphorylated AMP-activated protein kinase-α (pAMPKα), silent information regulator 2 homolog 1 (Sirt1), and peroxisome proliferator activated receptor gamma coactivator-1α (PGC1α). RSV attenuated CR-induced pAMPKα/AMPKαincreases. RSV increased levels of Sirt1 protein in the OF zebrafish, and decreased OF-induced increase in peroxisome proliferator-activated receptor-γ (PPARγ) protein level. Additionally, RSV down-regulated caveolin-1 and up-regulated microtubule-associated protein 1 light chain 3 -II (LC3-II) protein levels in OF zebrafish. In conclusion, these results suggest that 1) CR reduces plasma TG level through activation of the AMPKα-Sirt1- PGC1α pathway; 2) under different dietary stress conditions RSV might regulate AMPK phosphorylation bi-directionally; 3) RSV might regulate lipid metabolism through the AMPKα-Sirt1-PPARγ pathway in OF zebrafish.