Platinum nanoparticles induce damage to DNA and inhibit DNA replication

• Published in: PloS one 2017-07, Vol.12 (7), p.e0180798-e0180798
• Main Authors: Nejdl, Lukas, Kudr, Jiri, Moulick, Amitava, Hegerova, Dagmar, Ruttkay-Nedecky, Branislav, Gumulec, Jaromir, Cihalova, Kristyna, Smerkova, Kristyna, Dostalova, Simona, Krizkova, Sona, Novotna, Marie, Kopel, Pavel, Adam, Vojtech
• Format: Article
• Language: English
• Subjects: Absorption spectroscopy; Amplification; Antiinfectives and antibacterials; Atomic absorption analysis; Atomic absorption spectroscopy; Atomic structure; Bacteria; Biochemistry; Biocompatibility; Biology and Life Sciences; Cancer; Cell Line; Cells, Cultured; Chains (polymeric); Chemotherapy; Cisplatin; Cytotoxicity; Damage; Denaturation; Deoxyribonucleic acid; DNA; DNA biosynthesis; DNA Damage; DNA polymerase; DNA Replication; DNA sequencing; DNA structure; Encapsulation; Engineering and Technology; Erythrocytes; Erythrocytes - drug effects; Fibroblasts; Fibroblasts - drug effects; Fluorescence; Fragmentation; Gene sequencing; Growth curves; Humans; In vitro methods and tests; Light scattering; Liposomes; Medicine and Health Sciences; Metal Nanoparticles - adverse effects; Metal Nanoparticles - chemistry; Nanoparticles; Oxidative Stress; Phages; Photon correlation spectroscopy; Physical Sciences; Physiological aspects; Platinum; Platinum - adverse effects; Platinum - chemistry; Platinum compounds; Polymerase chain reaction; Protein structure; Quantum dots; Research and Analysis Methods; Secondary structure; Spectral analysis; Spectrometry; Spectrophotometry; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - genetics; X-ray fluorescence; Zeta potential
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0180798

Sparsely tested group of platinum nanoparticles (PtNPs) may have a comparable effect as complex platinum compounds. The aim of this study was to observe the effect of PtNPs in in vitro amplification of DNA fragment of phage λ, on the bacterial cultures (Staphylococcus aureus), human foreskin fibroblasts and erythrocytes. In vitro synthesized PtNPs were characterized by dynamic light scattering (PtNPs size range 4.8-11.7 nm), zeta potential measurements (-15 mV at pH 7.4), X-ray fluorescence, UV/vis spectrophotometry and atomic absorption spectrometry. The PtNPs inhibited the DNA replication and affected the secondary structure of DNA at higher concentrations, which was confirmed by polymerase chain reaction, DNA sequencing and DNA denaturation experiments. Further, cisplatin (CisPt), as traditional chemotherapy agent, was used in all parallel experiments. Moreover, the encapsulation of PtNPs in liposomes (LipoPtNPs) caused an approximately 2.4x higher of DNA damage in comparison with CisPt, LipoCisPt and PtNPs. The encapsulation of PtNPs in liposomes also increased their antibacterial, cytostatic and cytotoxic effect, which was determined by the method of growth curves on S. aureus and HFF cells. In addition, both the bare and encapsulated PtNPs caused lower oxidative stress (determined by GSH/GSSG ratio) in the human erythrocytes compared to the bare and encapsulated CisPt. CisPt was used in all parallel experiments as traditional chemotherapy agent.