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DNA barcoding of odonates from the Upper Plata basin: Database creation and genetic diversity estimation

We present a DNA barcoding study of Neotropical odonates from the Upper Plata basin, Brazil. A total of 38 species were collected in a transition region of "Cerrado" and Atlantic Forest, both regarded as biological hotspots, and 130 cytochrome c oxidase subunit I (COI) barcodes were genera...

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Published in:PloS one 2017-08, Vol.12 (8), p.e0182283-e0182283
Main Authors: Koroiva, Ricardo, Pepinelli, Mateus, Rodrigues, Marciel Elio, Roque, Fabio de Oliveira, Lorenz-Lemke, Aline Pedroso, Kvist, Sebastian
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description We present a DNA barcoding study of Neotropical odonates from the Upper Plata basin, Brazil. A total of 38 species were collected in a transition region of "Cerrado" and Atlantic Forest, both regarded as biological hotspots, and 130 cytochrome c oxidase subunit I (COI) barcodes were generated for the collected specimens. The distinct gap between intraspecific (0-2%) and interspecific variation (15% and above) in COI, and resulting separation of Barcode Index Numbers (BIN), allowed for successful identification of specimens in 94% of cases. The 6% fail rate was due to a shared BIN between two separate nominal species. DNA barcoding, based on COI, thus seems to be a reliable and efficient tool for identifying Neotropical odonate specimens down to the species level. These results underscore the utility of DNA barcoding to aid specimen identification in diverse biological hotspots, areas that require urgent action regarding taxonomic surveys and biodiversity conservation.
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A total of 38 species were collected in a transition region of "Cerrado" and Atlantic Forest, both regarded as biological hotspots, and 130 cytochrome c oxidase subunit I (COI) barcodes were generated for the collected specimens. The distinct gap between intraspecific (0-2%) and interspecific variation (15% and above) in COI, and resulting separation of Barcode Index Numbers (BIN), allowed for successful identification of specimens in 94% of cases. The 6% fail rate was due to a shared BIN between two separate nominal species. DNA barcoding, based on COI, thus seems to be a reliable and efficient tool for identifying Neotropical odonate specimens down to the species level. 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A total of 38 species were collected in a transition region of "Cerrado" and Atlantic Forest, both regarded as biological hotspots, and 130 cytochrome c oxidase subunit I (COI) barcodes were generated for the collected specimens. The distinct gap between intraspecific (0-2%) and interspecific variation (15% and above) in COI, and resulting separation of Barcode Index Numbers (BIN), allowed for successful identification of specimens in 94% of cases. The 6% fail rate was due to a shared BIN between two separate nominal species. DNA barcoding, based on COI, thus seems to be a reliable and efficient tool for identifying Neotropical odonate specimens down to the species level. 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subjects Accuracy
Animals
Bar codes
Biodiversity
Biology and life sciences
Brazil
Computer and information sciences
Conservation
Cytochrome
Cytochrome c
Cytochrome-c oxidase
Databases, Genetic
Deoxyribonucleic acid
DNA
DNA barcoding
DNA Barcoding, Taxonomic
Ecology
Ecology and Environmental Sciences
Electron Transport Complex IV - genetics
Evolutionary biology
Gene sequencing
Genetic diversity
Genetic Variation
Geography
Hot spots
Identification
Interspecific
Mitochondrial DNA
Morphology
Odonata
Odonata - genetics
Oxidase
People and places
Phylogeny
Research and analysis methods
Sequence Analysis, DNA
Species Specificity
Taxonomy
Wildlife conservation
title DNA barcoding of odonates from the Upper Plata basin: Database creation and genetic diversity estimation
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