Conformational risk factors of brachycephalic obstructive airway syndrome (BOAS) in pugs, French bulldogs, and bulldogs

• Published in: PloS one 2017-08, Vol.12 (8), p.e0181928-e0181928
• Main Authors: Liu, Nai-Chieh, Troconis, Eileen L, Kalmar, Lajos, Price, David J, Wright, Hattie E, Adams, Vicki J, Sargan, David R, Ladlow, Jane F
• Format: Article
• Language: English
• Subjects: Air flow; Airway Obstruction - diagnosis; Airway Obstruction - genetics; Airway Obstruction - veterinary; Animals; Biology and Life Sciences; Breeding; Craniosynostoses - diagnosis; Craniosynostoses - genetics; Craniosynostoses - veterinary; Diseases and pests; Dog Diseases - diagnosis; Dog Diseases - genetics; Dogs; Female; Male; Mathematical models; Medicine and Health Sciences; Neck; Nose; Obesity; Observer Variation; Physical Sciences; Plethysmography; Plethysmography, Whole Body; Regression Analysis; Reproducibility of Results; Research and Analysis Methods; Respiratory diseases; Respiratory function; Respiratory tract; Respiratory tract diseases; Risk analysis; Risk Factors; Skull; Sleep apnea; Species Specificity; Surgery; Syncope; Syndrome; Veterinary medicine; Weight control
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0181928

Extremely brachycephalic, or short-muzzled, dog breeds such as pugs, French bulldogs, and bulldogs are prone to the conformation-related respiratory disorder-brachycephalic obstructive airway syndrome (BOAS). Affected dogs present with a wide range of clinical signs from snoring and exercise intolerance, to life-threatening events such as syncope. In this study, conformational risk factors for BOAS that could potentially aid in breeding away from BOAS were sought. Six hundred and four pugs, French bulldogs, and bulldogs were included in the study. Soft tape measurements of the head and body were used and the inter-observer reproducibility was evaluated. Breed-specific models were developed to assess the associations between the conformational factors and BOAS status based on functional grading. The models were further validated by means of a BOAS index, which is an objective measurement of respiratory function using whole-body barometric plethysmography. The final models have good predictive power for discriminating BOAS (-) and BOAS (+) phenotypes indicated by the area under the curve values of >80% on the receiver operating curves. When other factors were controlled, stenotic nostrils were associated with BOAS in all three breeds; pugs and bulldogs with higher body condition scores (BCS) had a higher risk of developing BOAS. Among the standardized conformational measurements (i.e. craniofacial ratio (CFR), eye width ratio (EWR), skull index (SI), neck girth ratio (NGR), and neck length ratio (NLR)), for pugs EWR and SI, for French bulldogs NGR and NLR, and for bulldogs SI and NGR showed significant associations with BOAS status. However, the NGR in bulldogs was the only significant predictor that also had satisfactory inter-observer reproducibility. A NGR higher than 0.71 in male bulldogs was predictive of BOAS with approximately 70% sensitivity and specificity. In conclusion, stenotic nostrils, BCS, and NGR were found to be valid, easily applicable predictors for BOAS (+).