Sequence homology between HLA-bound cytomegalovirus and human peptides: A potential trigger for alloreactivity

Human cytomegalovirus (hCMV) reactivation may often coincide with the development of graft-versus-host-disease (GVHD) in stem cell transplantation (SCT). Seventy seven SCT donor-recipient pairs (DRP) (HLA matched unrelated donor (MUD), n = 50; matched related donor (MRD), n = 27) underwent whole exo...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2017-08, Vol.12 (8), p.e0178763-e0178763
Main Authors: Hall, Charles E, Koparde, Vishal N, Jameson-Lee, Maximilian, Elnasseh, Abdelrhman G, Scalora, Allison F, Kobulnicky, David J, Serrano, Myrna G, Roberts, Catherine H, Buck, Gregory A, Neale, Michael C, Nixon, Daniel E, Toor, Amir A
Format: Article
Language:English
Subjects:
Activation
Affinity
Amino acid sequence
Amino acids
Antigen-antibody reactions
Antigens
Biology and Life Sciences
Bone marrow
Cancer
Care and treatment
Clone Cells
Complications and side effects
Computational Biology
Cross Reactions - immunology
Cytomegalovirus
Cytomegalovirus - metabolism
Cytomegalovirus infections
Data bases
Development and progression
Graft vs Host Disease - immunology
Graft vs Host Disease - virology
Graft vs. host disease
Graft-versus-host reaction
Health aspects
Histocompatibility antigen HLA
HLA Antigens - chemistry
Homology
Humans
Immunogenicity
Infectious diseases
Leukemia
Libraries
Lymphocytes
Medicine and Health Sciences
Molecular Mimicry
Mud
Organs
Patients
Peptide libraries
Peptides
Peptides - chemistry
Proteome - metabolism
Research and Analysis Methods
Sequence Homology, Amino Acid
Single-nucleotide polymorphism
Stem cell transplantation
Stem cells
T-Lymphocytes - cytology
Transplantation
Transplants & implants
Viral infections
Viral Proteins - metabolism
Viremia
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human cytomegalovirus (hCMV) reactivation may often coincide with the development of graft-versus-host-disease (GVHD) in stem cell transplantation (SCT). Seventy seven SCT donor-recipient pairs (DRP) (HLA matched unrelated donor (MUD), n = 50; matched related donor (MRD), n = 27) underwent whole exome sequencing to identify single nucleotide polymorphisms (SNPs) generating alloreactive peptide libraries for each DRP (9-mer peptide-HLA complexes); Human CMV CROSS (Cross-Reactive Open Source Sequence) database was compiled from NCBI; HLA class I binding affinity for each DRPs HLA was calculated by NetMHCpan 2.8 and hCMV- derived 9-mers algorithmically compared to the alloreactive peptide-HLA complex libraries. Short consecutive (≥6) amino acid (AA) sequence homology matching hCMV to recipient peptides was considered for HLA-bound-peptide (IC50
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0178763