Effect of naringin on gp120-induced injury mediated by P2X7 receptors in rat primary cultured microglia

Human immunodeficiency virus type-1 (HIV-1) envelope glycoprotein 120 has been shown to activate microglia, causing release of inflammatory and toxic factors. The P2X7 receptor, primarily expressed on microglia, is closely associated with inflammation. Naringin, a plant bioflavonoid, has anti-inflam...

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Published in:PloS one 2017-08, Vol.12 (8), p.e0183688
Main Authors: Chen, Qiang, Wu, Hui, Tao, Jia, Liu, Chenglong, Deng, Zeyu, Liu, Yang, Chen, Guoqiao, Liu, Baoyun, Xu, Changshui
Format: Article
Language:English
Subjects:
Acquired immune deficiency syndrome
Adenosine Triphosphate - metabolism
AIDS
Animals
Apoptosis
Biology and Life Sciences
Blotting, Western
Cell injury
Cells, Cultured
Chemokines
Cytokines
Dementia
Disease
Flavanones - pharmacology
Gene expression
Glycoprotein gp120
Glycoproteins
HIV
HIV Envelope Protein gp120 - metabolism
Human immunodeficiency virus
Immunofluorescence
In Situ Nick-End Labeling
Infections
Inflammation
Injuries
Interleukin 1
Interleukin-1beta - metabolism
Kinases
Laboratory animals
Medicine and Health Sciences
Microglia
Microglia - drug effects
Microglia - metabolism
mRNA
Neurotoxicity
Pathogenesis
Physiology
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Receptors
Receptors, Purinergic P2X7 - genetics
Receptors, Purinergic P2X7 - metabolism
Research and Analysis Methods
RNA, Messenger - genetics
Rodents
Stimulation
Tumor necrosis factor
Tumor Necrosis Factor-alpha - metabolism
Tumor necrosis factor-TNF
Viruses
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cited_by cdi_FETCH-LOGICAL-c4418-fab887ab01d318e9ed1f5af18587c33bfd4dfe04dfead73cabf1e5122639bebf3
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container_issue 8
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Wu, Hui
Tao, Jia
Liu, Chenglong
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Liu, Yang
Chen, Guoqiao
Liu, Baoyun
Xu, Changshui
description Human immunodeficiency virus type-1 (HIV-1) envelope glycoprotein 120 has been shown to activate microglia, causing release of inflammatory and toxic factors. The P2X7 receptor, primarily expressed on microglia, is closely associated with inflammation. Naringin, a plant bioflavonoid, has anti-inflammatory and anti-oxidative properties. We hypothesized that P2X7 receptor mediated gp120-induced injury in primary cultured microglia, and that naringin would have a protective effect. We showed that HIV-1 gp120 peptide (V3 loop, fragment 308-331) appeared to induce apoptosis of primary cultured microglia. However, there was a decrease of microglia apoptosis in gp120+naringin group compared with gp120 group. Using qPCR, Western blot, and immunofluorescence, we showed that gp120 stimulated expression of P2X7 mRNA and receptor protein, and this stimulation was inhibited by naringin. Treatment with gp120 increased concentrations of eATP, TNFα and IL-1β, and these effects were inhibited by naringin. Taken together, these results suggested that gp120 contributed to microglial cell injury and neurotoxic activity by up-regulating expression of P2X7, in a naringin-protective manner.
doi_str_mv 10.1371/journal.pone.0183688
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subjects Acquired immune deficiency syndrome
Adenosine Triphosphate - metabolism
AIDS
Animals
Apoptosis
Biology and Life Sciences
Blotting, Western
Cell injury
Cells, Cultured
Chemokines
Cytokines
Dementia
Disease
Flavanones - pharmacology
Gene expression
Glycoprotein gp120
Glycoproteins
HIV
HIV Envelope Protein gp120 - metabolism
Human immunodeficiency virus
Immunofluorescence
In Situ Nick-End Labeling
Infections
Inflammation
Injuries
Interleukin 1
Interleukin-1beta - metabolism
Kinases
Laboratory animals
Medicine and Health Sciences
Microglia
Microglia - drug effects
Microglia - metabolism
mRNA
Neurotoxicity
Pathogenesis
Physiology
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Receptors
Receptors, Purinergic P2X7 - genetics
Receptors, Purinergic P2X7 - metabolism
Research and Analysis Methods
RNA, Messenger - genetics
Rodents
Stimulation
Tumor necrosis factor
Tumor Necrosis Factor-alpha - metabolism
Tumor necrosis factor-TNF
Viruses
title Effect of naringin on gp120-induced injury mediated by P2X7 receptors in rat primary cultured microglia
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