Enhancing antibacterial effect of sodium hypochlorite by low electric current-assisted sonic agitation

This research focused on the effects of low electric current (μE)-assisted sonic agitation of sodium hypochlorite on Enterococcus faecalis infected human root dentin. Extracted human canine roots were instrumented, sterilized, and experimentally contaminated with E. faecalis. After incubation for 21...

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Published in:PloS one 2017-08, Vol.12 (8), p.e0183895-e0183895
Main Authors: Maden, Murat, Ertuğrul, İhsan Furkan, Orhan, Ekim Onur, Erik, Cevat Emre, Yetiş, Ceylan Çağıl, Tuncer, Yasin, Kahriman, Mesud
Format: Article
Language:English
Subjects:
Agitation
Anti-Bacterial Agents - pharmacology
Bacteria
Biofilms
Biofilms - drug effects
Biology and Life Sciences
Canine teeth
Care and treatment
Colonies
Decontamination
Dental Pulp Cavity - microbiology
Dental roots
Dentin
Dentistry
Disinfectants - pharmacology
Electric currents
Electricity
Electron microscopy
Engineering and Technology
Enterococcal infections
Enterococcus faecalis - drug effects
Enterococcus faecalis - physiology
Gram-Positive Bacterial Infections - drug therapy
Health aspects
Humans
Irrigation
Lavage
Medicine and Health Sciences
Microorganisms
Periodontal diseases
Physical Sciences
Root Canal Therapy
Scanning electron microscopy
Sodium
Sodium hypochlorite
Sodium Hypochlorite - pharmacology
Sonication - methods
Teeth
Ultrasonic imaging
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cited_by cdi_FETCH-LOGICAL-c692t-c1daecd07d92dc6570dfd6fe0ffb0b1e2c08cd49d34e2044c82b5fe19df190d13
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container_end_page e0183895
container_issue 8
container_start_page e0183895
container_title PloS one
container_volume 12
creator Maden, Murat
Ertuğrul, İhsan Furkan
Orhan, Ekim Onur
Erik, Cevat Emre
Yetiş, Ceylan Çağıl
Tuncer, Yasin
Kahriman, Mesud
description This research focused on the effects of low electric current (μE)-assisted sonic agitation of sodium hypochlorite on Enterococcus faecalis infected human root dentin. Extracted human canine roots were instrumented, sterilized, and experimentally contaminated with E. faecalis. After incubation for 21 days, the presence of the biofilm was confirmed by scanning electron microscopy (n = 3). Roots were randomly divided into seven groups according to decontamination procedures: G1: no treatment; G2: sterile saline; G3: 5.25% sodium hypochlorite; G4: passive ultrasonic irrigation; G5: EndoActivator (Dentsply Tulsa Dental Specialties, Tulsa, OK) agitation (EA); G6: μE agitation; and G7: μE-assisted sonic agitation. Fixed μE amperage and intensities were applied in G6 and G7. Following microbial sampling, bacterial colonies were counted using the direct plating method. Biofilm was not eradicated in any sample. The μE-assisted sonic agitation of sodium hypochlorite revealed the lowest cfu values (p0.05). Based on available evidence, the following conclusions were drawn: The μE-assisted sonic agitation increased the antibiofilm efficiency of sodium hypochlorite than passive ultrasonic irrigation and EndoActivator. The μE-assisted sonic agitation on 5.25% sodium hypochlorite is not capable to eradicate biofilms at 10mA energy level in 60s.
doi_str_mv 10.1371/journal.pone.0183895
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Extracted human canine roots were instrumented, sterilized, and experimentally contaminated with E. faecalis. After incubation for 21 days, the presence of the biofilm was confirmed by scanning electron microscopy (n = 3). Roots were randomly divided into seven groups according to decontamination procedures: G1: no treatment; G2: sterile saline; G3: 5.25% sodium hypochlorite; G4: passive ultrasonic irrigation; G5: EndoActivator (Dentsply Tulsa Dental Specialties, Tulsa, OK) agitation (EA); G6: μE agitation; and G7: μE-assisted sonic agitation. Fixed μE amperage and intensities were applied in G6 and G7. Following microbial sampling, bacterial colonies were counted using the direct plating method. Biofilm was not eradicated in any sample. The μE-assisted sonic agitation of sodium hypochlorite revealed the lowest cfu values (p&lt;0.05), whereas there were no significant differences among the passive ultrasonic irrigation, EndoActivator and μE agitation alone (p&gt;0.05). 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subjects Agitation
Anti-Bacterial Agents - pharmacology
Bacteria
Biofilms
Biofilms - drug effects
Biology and Life Sciences
Canine teeth
Care and treatment
Colonies
Decontamination
Dental Pulp Cavity - microbiology
Dental roots
Dentin
Dentistry
Disinfectants - pharmacology
Electric currents
Electricity
Electron microscopy
Engineering and Technology
Enterococcal infections
Enterococcus faecalis - drug effects
Enterococcus faecalis - physiology
Gram-Positive Bacterial Infections - drug therapy
Health aspects
Humans
Irrigation
Lavage
Medicine and Health Sciences
Microorganisms
Periodontal diseases
Physical Sciences
Root Canal Therapy
Scanning electron microscopy
Sodium
Sodium hypochlorite
Sodium Hypochlorite - pharmacology
Sonication - methods
Teeth
Ultrasonic imaging
title Enhancing antibacterial effect of sodium hypochlorite by low electric current-assisted sonic agitation
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