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Plant flavones enhance antimicrobial activity of respiratory epithelial cell secretions against Pseudomonas aeruginosa
Flavones are a class of natural plant secondary metabolites that have anti-inflammatory and anti-bacterial effects. Some flavones also activate the T2R14 bitter taste receptor, which is expressed in motile cilia of the sinonasal epithelium and activates innate immune nitric oxide (NO) production. Fl...
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| Published in: | PloS one 2017-09, Vol.12 (9), p.e0185203-e0185203 |
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| creator | Hariri, Benjamin M McMahon, Derek B Chen, Bei Adappa, Nithin D Palmer, James N Kennedy, David W Lee, Robert J |
| description | Flavones are a class of natural plant secondary metabolites that have anti-inflammatory and anti-bacterial effects. Some flavones also activate the T2R14 bitter taste receptor, which is expressed in motile cilia of the sinonasal epithelium and activates innate immune nitric oxide (NO) production. Flavones may thus be potential therapeutics for respiratory infections. Our objective was to examine the anti-microbial effects of flavones on the common sinonasal pathogens Candida albicans, Staphylococcus aureus, and Pseudomonas aeruginosa, evaluating both planktonic and biofilm growth. Flavones had only very low-level antibacterial activity alone. They did not reduce biofilm formation, but did reduce production of the important P. aeruginosa inflammatory mediator and ciliotoxin pyocyanin. However, flavones exhibited synergy against P. aeruginosa in the presence of antibiotics or recombinant human lysozyme. They also enhanced the efficacy of antimicrobials secreted by cultured and primary human airway cells grown at air-liquid interface. This suggests that flavones may have anti-gram-negative potential as topical therapeutics when combined with antibiotics or in the context of innate antimicrobials secreted by the respiratory or other epithelia. This may have an additive effect when combined with T2R14-activated NO production. Additional studies are necessary to understand which flavone compounds or mixtures are the most efficacious. |
| doi_str_mv | 10.1371/journal.pone.0185203 |
| format | article |
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Some flavones also activate the T2R14 bitter taste receptor, which is expressed in motile cilia of the sinonasal epithelium and activates innate immune nitric oxide (NO) production. Flavones may thus be potential therapeutics for respiratory infections. Our objective was to examine the anti-microbial effects of flavones on the common sinonasal pathogens Candida albicans, Staphylococcus aureus, and Pseudomonas aeruginosa, evaluating both planktonic and biofilm growth. Flavones had only very low-level antibacterial activity alone. They did not reduce biofilm formation, but did reduce production of the important P. aeruginosa inflammatory mediator and ciliotoxin pyocyanin. However, flavones exhibited synergy against P. aeruginosa in the presence of antibiotics or recombinant human lysozyme. They also enhanced the efficacy of antimicrobials secreted by cultured and primary human airway cells grown at air-liquid interface. This suggests that flavones may have anti-gram-negative potential as topical therapeutics when combined with antibiotics or in the context of innate antimicrobials secreted by the respiratory or other epithelia. This may have an additive effect when combined with T2R14-activated NO production. Additional studies are necessary to understand which flavone compounds or mixtures are the most efficacious.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0185203</identifier><identifier>PMID: 28931063</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Anti-Bacterial Agents - pharmacology ; Antibacterial activity ; Antibiotics ; Antimicrobial activity ; Antimicrobial agents ; Bacteria ; Bacterial effects ; Biology and Life Sciences ; Bitter taste ; Bronchi - cytology ; Candida albicans - drug effects ; Care and treatment ; Cell Line ; Chinese medicine ; Cilia ; Drug Synergism ; Epithelial Cells - drug effects ; Epithelial Cells - microbiology ; Epithelial Cells - secretion ; Epithelium ; Flavones ; Flavones - pharmacology ; Flavonoids ; Genomes ; Gram-negative bacteria ; Health aspects ; Humans ; Inflammation ; Kinases ; Lysozyme ; Medicine and Health Sciences ; Metabolites ; Microbial Sensitivity Tests ; Microorganisms ; Muramidase - pharmacology ; Neck ; Nitric oxide ; Otolaryngology ; Paranasal Sinuses - cytology ; Physiological aspects ; Plants - chemistry ; Proteins ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - pathogenicity ; Pyocyanin ; Respiratory tract ; Respiratory tract diseases ; Secondary metabolites ; Secretions ; Staphylococcus aureus - drug effects ; Staphylococcus infections ; Surgery ; Taste receptors</subject><ispartof>PloS one, 2017-09, Vol.12 (9), p.e0185203-e0185203</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Hariri et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Some flavones also activate the T2R14 bitter taste receptor, which is expressed in motile cilia of the sinonasal epithelium and activates innate immune nitric oxide (NO) production. Flavones may thus be potential therapeutics for respiratory infections. Our objective was to examine the anti-microbial effects of flavones on the common sinonasal pathogens Candida albicans, Staphylococcus aureus, and Pseudomonas aeruginosa, evaluating both planktonic and biofilm growth. Flavones had only very low-level antibacterial activity alone. They did not reduce biofilm formation, but did reduce production of the important P. aeruginosa inflammatory mediator and ciliotoxin pyocyanin. However, flavones exhibited synergy against P. aeruginosa in the presence of antibiotics or recombinant human lysozyme. They also enhanced the efficacy of antimicrobials secreted by cultured and primary human airway cells grown at air-liquid interface. This suggests that flavones may have anti-gram-negative potential as topical therapeutics when combined with antibiotics or in the context of innate antimicrobials secreted by the respiratory or other epithelia. This may have an additive effect when combined with T2R14-activated NO production. Additional studies are necessary to understand which flavone compounds or mixtures are the most efficacious.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>28931063</pmid><doi>10.1371/journal.pone.0185203</doi><tpages>e0185203</tpages><orcidid>https://orcid.org/0000-0001-5826-6686</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2017-09, Vol.12 (9), p.e0185203-e0185203 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1940903199 |
| source | NCBI_PubMed Central（免费）; Publicly Available Content (ProQuest) |
| subjects | Anti-Bacterial Agents - pharmacology Antibacterial activity Antibiotics Antimicrobial activity Antimicrobial agents Bacteria Bacterial effects Biology and Life Sciences Bitter taste Bronchi - cytology Candida albicans - drug effects Care and treatment Cell Line Chinese medicine Cilia Drug Synergism Epithelial Cells - drug effects Epithelial Cells - microbiology Epithelial Cells - secretion Epithelium Flavones Flavones - pharmacology Flavonoids Genomes Gram-negative bacteria Health aspects Humans Inflammation Kinases Lysozyme Medicine and Health Sciences Metabolites Microbial Sensitivity Tests Microorganisms Muramidase - pharmacology Neck Nitric oxide Otolaryngology Paranasal Sinuses - cytology Physiological aspects Plants - chemistry Proteins Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - pathogenicity Pyocyanin Respiratory tract Respiratory tract diseases Secondary metabolites Secretions Staphylococcus aureus - drug effects Staphylococcus infections Surgery Taste receptors |
| title | Plant flavones enhance antimicrobial activity of respiratory epithelial cell secretions against Pseudomonas aeruginosa |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T14%3A18%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Plant%20flavones%20enhance%20antimicrobial%20activity%20of%20respiratory%20epithelial%20cell%20secretions%20against%20Pseudomonas%20aeruginosa&rft.jtitle=PloS%20one&rft.au=Hariri,%20Benjamin%20M&rft.date=2017-09-20&rft.volume=12&rft.issue=9&rft.spage=e0185203&rft.epage=e0185203&rft.pages=e0185203-e0185203&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0185203&rft_dat=%3Cgale_plos_%3EA505563354%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c758t-e1504876ac3f4512c5500170e9a34c03e57c5ec766ddcb42b7b56a2bd91b033a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1940903199&rft_id=info:pmid/28931063&rft_galeid=A505563354&rfr_iscdi=true |