Characterization of the nasal and oral microbiota of detection dogs

Little is known about physiological factors that affect the sense of olfaction in dogs. The objectives of this study were to describe the canine nasal and oral microbiota in detection dogs. We sought to determine the bacterial composition of the nasal and oral microbiota of a diverse population of d...

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Bibliographic Details
Published in:PloS one 2017-09, Vol.12 (9), p.e0184899-e0184899
Main Authors: Isaiah, Anitha, Hoffmann, Aline Rodrigues, Kelley, Russ, Mundell, Paul, Steiner, Jörg M, Suchodolski, Jan S
Format: Article
Language:English
Subjects:
Analysis
Animals
Bacteria
Biodiversity
Bioinformatics
Biology and Life Sciences
Cohort Studies
Communities
Deoxyribonucleic acid
Discriminant Analysis
DNA
Dogs
Dogs - microbiology
Drug and Narcotic Control
Explosive Agents
Explosives
Explosives detection
Female
Gene expression
Genomes
Laboratories
Law enforcement
Linear Models
Male
Medicine and Health Sciences
Methods
Microbiota (Symbiotic organisms)
Microbiota - genetics
Microorganisms
Military Personnel
Mouth - microbiology
Narcotics
Nose - microbiology
Olfaction
Pets - microbiology
Physiological aspects
Physiological effects
Physiological factors
Physiology
Police
Research and Analysis Methods
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
rRNA
Skin
Smell
Studies
Time Factors
Veterinary colleges
Veterinary medicine
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Summary:Little is known about physiological factors that affect the sense of olfaction in dogs. The objectives of this study were to describe the canine nasal and oral microbiota in detection dogs. We sought to determine the bacterial composition of the nasal and oral microbiota of a diverse population of detection canines. Nasal and oral swabs were collected from healthy dogs (n = 81) from four locations-Alabama, Georgia, California, and Texas. Nasal and oral swabs were also collected from a second cohort of detection canines belonging to three different detection job categories: explosive detection dogs (SP-E; n = 22), patrol and narcotics detection dogs (P-NDD; n = 15), and vapor wake dogs (VWD-E; n = 9). To understand if the nasal and oral microbiota of detection canines were variable, sample collection was repeated after 7 weeks in a subset of dogs. DNA was extracted from the swabs and used for 454-pyrosequencing of the16S rRNA genes. Nasal samples had a significantly lower diversity than oral samples (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0184899