A novel miniature in-line load-cell to measure in-situ tensile forces in the tibialis anterior tendon of rats

• Published in: PloS one 2017-09, Vol.12 (9), p.e0185209-e0185209
• Main Authors: Schmoll, Martin, Unger, Ewald, Bijak, Manfred, Stoiber, Martin, Lanmüller, Hermann, Jarvis, Jonathan Charles
• Format: Article
• Language: English
• Subjects: Animals; Biology and Life Sciences; Biomechanical Phenomena; Biomechanics; Biomedical engineering; Biosensors; Computer and Information Sciences; Controlled conditions; Deformation; Deformation mechanisms; Engineering; Engineering and Technology; Health physics; Laboratory animals; Linearity; Load cells; Male; Materials Testing - instrumentation; Medicine and Health Sciences; Methods; Miniaturization - instrumentation; Muscles; Musculoskeletal system; Optics; Physical Sciences; Physiological aspects; Rats; Rats, Wistar; Research and Analysis Methods; Rodents; Tendons; Tendons - physiology; Tensile Strength; Terminology; Tibia; Weight-Bearing
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0185209

Direct measurements of muscular forces usually require a substantial rearrangement of the biomechanical system. To circumvent this problem, various indirect techniques have been used in the past. We introduce a novel direct method, using a lightweight (~0.5 g) miniature (3 x 3 x 7 mm) in-line load-cell to measure tension in the tibialis anterior tendon of rats. A linear motor was used to produce force-profiles to assess linearity, step-response, hysteresis and frequency behavior under controlled conditions. Sensor responses to a series of rectangular force-pulses correlated linearly (R2 = 0.999) within the range of 0-20 N. The maximal relative error at full scale (20 N) was 0.07% of the average measured signal. The standard deviation of the mean response to repeated 20 N force pulses was ± 0.04% of the mean response. The step-response of the load-cell showed the behavior of a PD2T2-element in control-engineering terminology. The maximal hysteretic error was 5.4% of the full-scale signal. Sinusoidal signals were attenuated maximally (-4 dB) at 200 Hz, within a measured range of 0.01-200 Hz. When measuring muscular forces this should be of minor concern as the fusion-frequency of muscles is generally much lower. The newly developed load-cell measured tensile forces of up to 20 N, without inelastic deformation of the sensor. It qualifies for various applications in which it is of interest directly to measure forces within a particular tendon causing only minimal disturbance to the biomechanical system.