Adenoviral vector mediated ferritin over-expression in mesenchymal stem cells detected by 7T MRI in vitro

• Published in: PloS one 2017-09, Vol.12 (9), p.e0185260-e0185260
• Main Authors: Dai, Hai-Yang, He, Rong, Zhang, Ying, Wu, Ren-Hua, Xiao, Ye-Yu
• Format: Article
• Language: English
• Subjects: Adenoviridae - genetics; Animals; Apoferritins - genetics; Apoferritins - metabolism; Biology and Life Sciences; Bone marrow; Cancer; Cell Differentiation; Cell Proliferation; Cell Survival; Cells, Cultured; Change detection; Correlation; Correlation analysis; Differentiation; Enzyme-Linked Immunosorbent Assay; Feasibility Studies; Ferritin; Gene therapy; Genetic aspects; Genetic Vectors; Health aspects; Homeostasis; Humans; Immunocytochemistry; Immunohistochemistry; Iron; Magnetic resonance imaging; Magnetic Resonance Imaging - methods; Medical imaging; Medicine and Health Sciences; Mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Mesenchyme; Mice; Overexpression; Physiological aspects; Plasmids; Pluripotency; Proteins; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Research and Analysis Methods; Stem cells; Up-Regulation; Vectors (Biology)
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0185260

The aim of the present work was to verify whether adenoviral vector mediated ferritin over-expression in mesenchymal stem cells could be detected by 7T MRI device, and to explore the relationship between ferritin content and MRI signal intensities. A recombined adenoviral vector (rAdV) encoding ferritin heavy chain (FTH1) subunit was specially designed for the aim of infecting bone marrow mesenchymal stem cells (BMSCs). Ferritin over-expression in BMSCs was determined by cell immunocytochemistry and the ferritin content in cells was determined by ELISA assay. BMSCs were subjected to cell viability, proliferation and multi-differentiation analyses as well as 7T MRI test using fast spin-echo pulse sequence. The R2 value andδR2 were calculated according to T2 mapping images. As was confirmed by cell immunocytochemistry and ELISA assay, rAdV mediated ferritin was over-expressed in BMSCs. Ferritin over-expression did not interfere with stem cell viability or pluripotent differentiation but slowed cell proliferation. The R2 value of BMSCs-FTH1 vs control BMSCs from 1-4 weeks was16.65±1.28 s-1 vs 13.99±0.80 s-1, (t = 3.94, p = 0.004), 15.63±1.37 s-1 vs 13.87±0.83 s-1 (t = 2.47, p = 0.039), 15.53±0.88 s-1 vs 14.25±0.53 s-1 (t = 2.80, p = 0.023) and 14.61±1.28 s-1 vs 13.69±1.03 s-1 (t = 1.25, p = 0.24), respectively. δR2 gradually decreased from 1-4 weeks and the difference between the groups had statistical significance (F = 12.45, p