Diet alters performance and transcription patterns in Oedaleus asiaticus (Orthoptera: Acrididae) grasshoppers

• Published in: PloS one 2017-10, Vol.12 (10), p.e0186397-e0186397
• Main Authors: Huang, Xunbing, Whitman, Douglas W, Ma, Jingchuan, McNeill, Mark Richard, Zhang, Zehua
• Format: Article
• Language: English
• Subjects: Acrididae; Analysis; Animals; Aphidoidea; Biochemistry; Biology; Biology and Life Sciences; Biosynthesis; Cluster Analysis; Deoxyribonucleic acid; Detoxification; Diet; DNA; DNA biosynthesis; DNA replication; Down-Regulation; Enzymes; Feeding; Feeding Behavior; Food; Gene expression; Genes; Grasses; Grasshoppers; Grasshoppers - genetics; Grasshoppers - physiology; Grasslands; Health aspects; Herbivores; Laboratories; Lipids; Locusta migratoria; Medicine and Health Sciences; Metabolism; Nutrition; Nutrition research; Nymph - metabolism; Oedaleus asiaticus; Orthoptera; Orthopterans; Phenotypic plasticity; Physiology; Plant diseases; Poaceae - growth & development; Proteins; RNA - chemistry; RNA - isolation & purification; RNA - metabolism; Sequence Analysis, DNA; Stress; Stresses; Transcription; Transcriptome; Up-Regulation
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0186397

We reared Oedaleus asiaticus grasshoppers under four different single-plant diets to examine the relationships among diet, performance, stress, and transcription patterns. Grasshoppers fed only Artemisia frigida (Asteraceae) were stressed, as indicated by their lower growth, size, development, and survival, in comparison to grasshoppers fed on any of three grasses, Cleistogenes squarrosa, Leymus chinensis, or Stipa krylovii (all Poaceae). We then used transcriptome analysis to examine how gene expression levels in O. asiaticus were altered by feeding on these diets. Nymphs fed A. frigida had the largest variation in gene expression profiles with a total of 299 genes significantly up- or down-regulated compared to those feeding on the three grasses: down-regulated genes included those involved in cuticle biosynthesis, DNA replication, biosynthesis and metabolism of nutrition. The up-regulated genes included stress-resistant and detoxifying enzymes. GO and KEGG enrichment analysis also showed that feeding on A. frigida could down-regulate biosynthesis and metabolism related pathways, and up-regulate stress-resistant and detoxification terms and pathways. Our results show that diet significantly altered gene-expression, and that unfavorable, stressful diets induce more transcriptional changes than favorable diets. Altered gene-expression represents phenotypic plasticity, and many such changes appear to be evolved, adaptive responses. The ease and regularity by which individuals shift phenotypes via altered transcription suggests that populations consist not of similar, fixed phenotypes, but of a collection of ever-changing, divergent phenotypes.