Hydrogen peroxide yields mechanistic insights into human mRNA capping enzyme function

• Published in: PloS one 2017-10, Vol.12 (10), p.e0186423-e0186423
• Main Authors: Mullen, Nicholas J, Price, David H
• Format: Article
• Language: English
• Subjects: Base Sequence; Biocatalysis; Biochemistry; Biology and Life Sciences; Capping; Cytomegalovirus; DNA-directed RNA polymerase; Enzyme Inhibitors - pharmacology; Enzymes; Gene expression; Humans; Hydrogen; Hydrogen ion concentration; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; Mammals; Mass spectrometry; Mass spectroscopy; Messenger RNA; Models, Molecular; mRNA capping enzyme; mRNA guanylyltransferase; mRNA processing; Nucleoside-Triphosphatase - antagonists & inhibitors; Nucleoside-Triphosphatase - chemistry; Nucleoside-Triphosphatase - metabolism; Nucleotidyltransferases - antagonists & inhibitors; Nucleotidyltransferases - chemistry; Nucleotidyltransferases - metabolism; Physical Sciences; Polymerase; Protein Domains; Proteins; Research and Analysis Methods; Ribonucleic acid; RNA; RNA Caps - genetics; RNA Caps - metabolism; RNA polymerase; RNA polymerase II; Transcription; Transcription (Genetics); Triphosphatase
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0186423

Capping of nascent RNA polymerase II (Pol II) transcripts is required for gene expression and the first two steps are catalyzed by separate 5' triphosphatase and guanylyltransferase activities of the human capping enzyme (HCE). The cap is added co-transcriptionally, but how the two activities are coordinated is unclear. Our previous in vitro work has suggested that an unidentified factor modulates the minimum length at which nascent transcripts can be capped. Using the same well-established in vitro system with hydrogen peroxide as a capping inhibitor, we show that this unidentified factor targets the guanylyltransferase activity of HCE. We also uncover the mechanism of HCE inhibition by hydrogen peroxide, and by using mass spectrometry demonstrate that the active site cysteine residue of the HCE triphosphatase domain becomes oxidized. Using recombinant proteins for the two separated HCE domains, we provide evidence that the triphosphatase normally acts on transcripts shorter than can be acted upon by the guanylyltransferase. Our further characterization of the capping reaction dependence on transcript length and its interaction with the unidentified modulator of capping raises the interesting possibility that the capping reaction could be regulated.