Effects of stand age and soil properties on soil bacterial and fungal community composition in Chinese pine plantations on the Loess Plateau

• Published in: PloS one 2017-10, Vol.12 (10), p.e0186501-e0186501
• Main Authors: Dang, Peng, Yu, Xuan, Le, Hien, Liu, Jinliang, Shen, Zhen, Zhao, Zhong
• Format: Article
• Language: English
• Subjects: Abundance; Afforestation; Age; Agricultural land; Analysis; Bacteria; Bacteria - classification; Bacteria - isolation & purification; Biochemistry; Biology; Biology and Life Sciences; Carbon; China; Community composition; Community development; Community structure; Drought; Earth Sciences; Ecology and Environmental Sciences; Ecosystem biology; Ecosystems; Environmental aspects; Environmental restoration; Forestry; Forests; Fungi; Fungi - classification; Fungi - isolation & purification; Hydrocarbons; Laboratories; Loess; Microbiology; Microorganisms; Next-generation sequencing; Nutrients; Nutrients in soil; Old fields; Pine; Pine trees; Pinus - microbiology; Plant communities; Plant diversity; Plantations; Rain; Soil; Soil carbon; Soil erosion; Soil Microbiology; Soil microorganisms; Soil nutrients; Soil properties; Soil sciences; Soil structure; Taxa; Technology assessment
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0186501

The effects of Chinese pine (Pinus tabuliformis) on soil variables after afforestation have been established, but microbial community changes still need to be explored. Using high-throughput sequencing technology, we analyzed bacterial and fungal community composition and diversity in soils from three stands of different-aged, designated 12-year-old (PF1), 29-year-old (PF2), and 53-year-old (PF3), on a Chinese pine plantation and from a natural secondary forest (NSF) stand that was almost 80 years old. Abandoned farmland (BL) was also analyzed. Shannon index values of both bacterial and fungal community in PF1 were greater than those in PF2, PF3 and NSF. Proteobacteria had the lowest abundance in BL, and the abundance increased with stand age. The abundance of Actinobacteria was greater in BL and PF1 soils than those in other sites. Among fungal communities, the dominant taxa were Ascomycota in BL and PF1 and Basidiomycota in PF2, PF3 and NSF, which reflected the successional patterns of fungal communities during the development of Chinese pine plantations. Therefore, the diversity and dominant taxa of soil microbial community in stands 12 and 29 years of age appear to have undergone significant changes; afterward, the soil microbial community achieved a relatively stable state. Furthermore, the abundances of the most dominant bacterial and fungal communities correlated significantly with organic C, total N, C:N, available N, and available P, indicating the dependence of these microbes on soil nutrients. Overall, our findings suggest that the large changes in the soil microbial community structure of Chinese pine plantation forests may be attributed to the phyla present (e.g., Proteobacteria, Actinobacteria, Ascomycota and Basidiomycota) which were affected by soil carbon and nutrients in the Loess Plateau.