The green tea modulates large intestinal microbiome and exo/endogenous metabolome altered through chronic UVB-exposure

• Published in: PloS one 2017-11, Vol.12 (11), p.e0187154-e0187154
• Main Authors: Jung, Eun Sung, Park, Hye Min, Hyun, Seung Min, Shon, Jong Cheol, Singh, Digar, Liu, Kwang-Hyeon, Whon, Tae Woong, Bae, Jin-Woo, Hwang, Jae Sung, Lee, Choong Hwan
• Format: Article
• Language: English
• Subjects: Acids; Amino acids; Analysis; Animals; Bacilli; Bases (nucleic acids); Bile; Bile acids; Biology and Life Sciences; Biotechnology; Body Weight - radiation effects; Carbohydrates; Catechin; Catechin - analysis; Diet; Dietary Supplements; Digestive system; Digestive tract; Eating - radiation effects; Exposure; Fatty acids; Female; Food; Gas Chromatography-Mass Spectrometry; Gastrointestinal Microbiome - radiation effects; Gastrointestinal tract; Genetic engineering; Green tea; Intestinal microflora; Intestine, Large - metabolism; Intestine, Large - microbiology; Intestine, Large - radiation effects; Large intestine; Lipids; Liver; Liver - metabolism; Medicine and Health Sciences; Metabolic Networks and Pathways - radiation effects; Metabolism; Metabolites; Metabolome - radiation effects; Metabolomics; Mice; Microbiota (Symbiotic organisms); Oxidative stress; Pharmaceutical sciences; Pharmacy; Physical sciences; Physiological aspects; Polyphenols; Properties; Radiation; Radiation damage; Radiation effects; Rodents; Skin; Taxa; Tea; Tea - chemistry; Ultraviolet radiation; Ultraviolet Rays
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0187154

The attenuating effects of green tea supplements (GTS) against the ultraviolet (UV) radiation induced skin damages are distinguished. However, the concomitant effects of GTS on the large intestinal microbiomes and associated metabolomes are largely unclear. Herein, we performed an integrated microbiome-metabolome analysis to uncover the esoteric links between gut microbiome and exo/endogenous metabolome maneuvered in the large intestine of UVB-exposed mice subjected to dietary GTS. In UVB-exposed mice groups (UVB), class Bacilli and order Bifidobacteriales were observed as discriminant taxa with decreased lysophospholipid levels compared to the unexposed mice groups subjected to normal diet (NOR). Conversely, in GTS fed UVB-exposed mice (U+GTS), the gut-microbiome diversity was greatly enhanced with enrichment in the classes, Clostridia and Erysipelotrichia, as well as genera, Allobaculum and Lachnoclostridium. Additionally, the gut endogenous metabolomes changed with an increase in amino acids, fatty acids, lipids, and bile acids contents coupled with a decrease in nucleobases and carbohydrate levels. The altered metabolomes exhibited high correlations with GTS enriched intestinal microflora. Intriguingly, the various conjugates of green tea catechins viz., sulfated, glucuronided, and methylated ones including their exogenous derivatives were detected from large intestinal contents and liver samples. Hence, we conjecture that the metabolic conversions for the molecular components in GTS strongly influenced the gut micro-environment in UVB-exposed mice groups, ergo modulate their gut-microbiome as well as exo/endogenous metabolomes.