Profiling of proteins secreted in the bovine oviduct reveals diverse functions of this luminal microenvironment

The oviductal microenvironment is a site for key events that involve gamete maturation, fertilization and early embryo development. Secretions into the oviductal lumen by either the lining epithelium or by transudation of plasma constituents are known to contain elements conducive for reproductive s...

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Published in:PloS one 2017-11, Vol.12 (11), p.e0188105-e0188105
Main Authors: Pillai, Viju Vijayan, Weber, Darren M, Phinney, Brett S, Selvaraj, Vimal
Format: Article
Language:English
Subjects:
Animal reproduction
Animal sciences
Apolipoproteins
Bioinformatics
Biology and Life Sciences
Bovidae
Breeding success
Cattle
Cell cycle
Embryonic development
Embryos
Epithelial cells
Epithelium
Extracellular matrix
Fertility
Fertilization
Functional analysis
Gene expression
Genomes
Growth factors
Homeostasis
Immunomodulation
In vitro fertilization
Kinases
Life sciences
Machinery and equipment
Maturation
Medicine and Health Sciences
Metabolism
Modulators
Ovaries
Oviduct
Penicillin
Physiological aspects
Proteins
Proteomics
Regulators
Reproduction
Rodents
Secretions
Sperm
Stem cells
Studies
Zoology
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description The oviductal microenvironment is a site for key events that involve gamete maturation, fertilization and early embryo development. Secretions into the oviductal lumen by either the lining epithelium or by transudation of plasma constituents are known to contain elements conducive for reproductive success. Although previous studies have identified some of these factors involved in reproduction, knowledge of secreted proteins in the oviductal fluid remains rudimentary with limited definition of function even in extensively studied species like cattle. In this study, we used a shotgun proteomics approach followed by bioinformatics sequence prediction to identify secreted proteins present in the bovine oviductal fluid (ex vivo) and secretions from the bovine oviductal epithelial cells (in vitro). From a total of 2087 proteins identified, 266 proteins could be classified as secreted, 109 (41%) of which were common for both in vivo and in vitro conditions. Pathway analysis indicated different classes of proteins that included growth factors, metabolic regulators, immune modulators, enzymes, and extracellular matrix components. Functional analysis revealed mechanisms in the oviductal lumen linked to immune homeostasis, gamete maturation, fertilization and early embryo development. These results point to several novel components that work together with known elements mediating functional homeostasis, and highlight the diversity of machinery associated with oviductal physiology and early events in cattle fertility.
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subjects Animal reproduction
Animal sciences
Apolipoproteins
Bioinformatics
Biology and Life Sciences
Bovidae
Breeding success
Cattle
Cell cycle
Embryonic development
Embryos
Epithelial cells
Epithelium
Extracellular matrix
Fertility
Fertilization
Functional analysis
Gene expression
Genomes
Growth factors
Homeostasis
Immunomodulation
In vitro fertilization
Kinases
Life sciences
Machinery and equipment
Maturation
Medicine and Health Sciences
Metabolism
Modulators
Ovaries
Oviduct
Penicillin
Physiological aspects
Proteins
Proteomics
Regulators
Reproduction
Rodents
Secretions
Sperm
Stem cells
Studies
Zoology
title Profiling of proteins secreted in the bovine oviduct reveals diverse functions of this luminal microenvironment
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