ChrASO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction

The chromate efflux pump encoding gene chrASO was identified on the chromosome of Shewanella oneidensis MR1. Although chrASO is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant ΔchrASO exhibits a chromate sensitive phenotype compared...

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Published in:PloS one 2017, Vol.12 (11), p.e0188516
Main Authors: Baaziz, Hiba, Gambari, Cyril, Boyeldieu, Anne, Ali Chaouche, Amine, Alatou, Radia, Méjean, Vincent, Jourlin-Castelli, Cécile, Fons, Michel
Format: Article
Language:English
Subjects:
Amino acids
Bacteria
Bacteriology
Biochemistry, Molecular Biology
Biology and life sciences
Burkholderia
Cell survival
Chromate
Chromates
Chromium
Cloning
Computer and Information Sciences
Deoxyribonucleic acid
DNA
E coli
Efflux
Escherichia coli
Genes
Genomics
Life Sciences
Microbiology and Parasitology
Physical Sciences
Proteins
Reduction
Research and analysis methods
Survival
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Summary:The chromate efflux pump encoding gene chrASO was identified on the chromosome of Shewanella oneidensis MR1. Although chrASO is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant ΔchrASO exhibits a chromate sensitive phenotype compared to that of the wild-type strain. Interestingly, heterologous expression of chrASO in E. coli confers resistance to high chromate concentration. Moreover, expression of chrASO in S. oneidensis and E. coli significantly improves Cr(VI) reduction. This effect could result either from extracytoplasmic chromate reduction or from a better cell survival leading to enhanced Cr(VI) reduction.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0188516