An attempt to interpret a biochemical mechanism of C4 photosynthetic thermo-tolerance under sudden heat shock on detached leaf in elevated CO2 grown maize

• Published in: PloS one 2017, Vol.12 (12), p.e0187437-e0187437
• Main Authors: Qu, Mingnan, Bunce, James A, Sicher, Richard C, Zhu, Xiaocen, Gao, Bo, Chen, Genyun
• Format: Article
• Language: English
• Subjects: Abiotic stress; Adaptation, Physiological; Assimilation; Biology and Life Sciences; Carbon dioxide; Carbon Dioxide - metabolism; Carbon dioxide effects; Citric acid; Citric Acid Cycle; Corn; Correlation analysis; Earth Sciences; Ecology and Environmental Sciences; Electrolyte leakage; Engineering and Technology; Enzymatic activity; Enzyme activity; Heat; Heat shock; Heat stress; Heat tolerance; Heat-Shock Response; Hexoses; High temperature; Inbreeding; Irradiance; Laboratories; Leaves; Metabolism; Metabolites; Mitigation; NADP; Open top; Photosynthesis; Physical Sciences; Physiology; Plant mitochondria; Plant sciences; Plants (botany); Research and Analysis Methods; RNA, Messenger - genetics; Seeds; Self-propagating synthesis; Solar irradiance; Starch; Studies; Temperature effects; Temperature tolerance; Thermal stability; Transcription; Tricarboxylic acid cycle; Zea mays; Zea mays - genetics; Zea mays - metabolism; Zea mays - physiology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0187437

Detached leaves at top canopy structures always experience higher solar irradiance and leaf temperature under natural conditions. The ability of tolerance to high temperature represents thermotolerance potential of whole-plants, but was less of concern. In this study, we used a heat-tolerant (B76) and a heat-susceptible (B106) maize inbred line to assess the possible mitigation of sudden heat shock (SHS) effects on photosynthesis (PN) and C4 assimilation pathway by elevated [CO2]. Two maize lines were grown in field-based open top chambers (OTCs) at ambient and elevated (+180 ppm) [CO2]. Top-expanded leaves for 30 days after emergence were suddenly exposed to a 45°C SHS for 2 hours in midday during measurements. Analysis on thermostability of cellular membrane showed there was 20% greater electrolyte leakage in response to the SHS in B106 compared to B76, in agreement with prior studies. Elevated [CO2] protected PN from SHS in B76 but not B106. The responses of PN to SHS among the two lines and grown CO2 treatments were closely correlated with measured decreases of NADP-ME enzyme activity and also to its reduced transcript abundance. The SHS treatments induced starch depletion, the accumulation of hexoses and also disrupted the TCA cycle as well as the C4 assimilation pathway in the both lines. Elevated [CO2] reversed SHS effects on citrate and related TCA cycle metabolites in B106 but the effects of elevated [CO2] were small in B76. These findings suggested that heat stress tolerance is a complex trait, and it is difficult to identify biochemical, physiological or molecular markers that accurately and consistently predict heat stress tolerance.