Microsatellite instability in chicken lymphoma induced by gallid herpesvirus 2

Microsatellite instability (MSI) has been found in a range of human tumors, and little is known of the links between MSI and herpesvirus. In order to investigate the relationship between MSI and Gallid herpesvirus 2 (GaHV-2)-induced lymphoma, fifteen Marek's disease (MD) lymphomas were analyzed...

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Bibliographic Details
Published in:PloS one 2013-07, Vol.8 (7), p.e68058-e68058
Main Authors: Yao, Da-Wei, Xu, Jia-Rong, Zhou, Zhen-Lei, Li, Shang-Tong, Yang, De-Ji
Format: Article
Language:English
Subjects:
Animal tissues
Animals
Annealing
Biology
Cancer
Chickens
Deoxyribonucleic acid
Development and progression
Disease
DNA
Electrophoresis, Polyacrylamide Gel
Fibroblasts
Gel electrophoresis
Gene Frequency
Genetic aspects
Genetic markers
Herpes viruses
Herpesvirus 2, Gallid - growth & development
Herpesvirus 2, Gallid - physiology
Host-Pathogen Interactions
Infections
Laboratory animals
Lymphoma
Lymphoma - genetics
Lymphoma - pathology
Lymphoma - virology
Marek Disease - genetics
Marek Disease - pathology
Marek Disease - virology
Marek's disease
Markers
Medical research
Medicine
Microsatellite Instability
Microsatellite Repeats - genetics
Microsatellites
Mutation
Non-Hodgkin's lymphomas
Pathogens
Physiological aspects
Polymerase Chain Reaction
Poultry
Proteins
Satellite DNA
Signal transduction
Stability
Tumors
Veterinary colleges
Veterinary medicine
Veterinary Science
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Summary:Microsatellite instability (MSI) has been found in a range of human tumors, and little is known of the links between MSI and herpesvirus. In order to investigate the relationship between MSI and Gallid herpesvirus 2 (GaHV-2)-induced lymphoma, fifteen Marek's disease (MD) lymphomas were analyzed through using 46 microsatellite markers, which were amplified by PCR from DNA specimens of lymphoma and normal muscular tissues from the same chicken. PCR products were evaluated by denaturing polyacrylamide gel electrophoresis for MSI analysis. MSI was proved in all lymphomas, at least in one locus. Thirty of the 46 microsatellite markers had microsatellite alterations. These results suggested that GaHV-2-induced lymphoma in chickens is related to MSI, and this is the first report to demonstrate that MSI is associated with the GaHV-2 induced lymphoma in chicken.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0068058