Genetic differentiation of the mitochondrial cytochrome oxidase C subunit I gene in genus Paramecium (Protista, Ciliophora)

The mitochondrial cytochrome c oxidase subunit I (COI) gene is being used increasingly for evaluating inter- and intra-specific genetic diversity of ciliated protists. However, very few studies focus on assessing genetic divergence of the COI gene within individuals and how its presence might affect...

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Bibliographic Details
Published in:PloS one 2013-10, Vol.8 (10), p.e77044-e77044
Main Authors: Zhao, Yan, Gentekaki, Eleni, Yi, Zhenzhen, Lin, Xiaofeng
Format: Article
Language:English
Subjects:
Base Sequence
Biodiversity
China
Cloning
COI protein
Cytochrome
Cytochrome-c oxidase
Deoxyribonucleic acid
Divergence
DNA
DNA, Mitochondrial - chemistry
DNA, Mitochondrial - genetics
DNA, Protozoan - chemistry
DNA, Protozoan - genetics
Electron Transport Complex IV - classification
Electron Transport Complex IV - genetics
Evolution
Genes
Genes, Mitochondrial - genetics
Genetic diversity
Genetic Variation
Geography
Haplotypes
Laboratories
Life sciences
Mitochondria
Mitochondrial DNA
Molecular Sequence Data
Morphology
Oxidase
Paramecium
Paramecium - classification
Paramecium - genetics
Paramecium bursaria
Paramecium caudatum
Paramecium duboscqui
Paramecium nephridiatum
Phylogenetics
Phylogeny
Plant diversity
Population structure
Protozoan Proteins - genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Species
Species diversity
Species Specificity
Studies
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Summary:The mitochondrial cytochrome c oxidase subunit I (COI) gene is being used increasingly for evaluating inter- and intra-specific genetic diversity of ciliated protists. However, very few studies focus on assessing genetic divergence of the COI gene within individuals and how its presence might affect species identification and population structure analyses. We evaluated the genetic variation of the COI gene in five Paramecium species for a total of 147 clones derived from 21 individuals and 7 populations. We identified a total of 90 haplotypes with several individuals carrying more than one haplotype. Parsimony network and phylogenetic tree analyses revealed that intra-individual diversity had no effect in species identification and only a minor effect on population structure. Our results suggest that the COI gene is a suitable marker for resolving inter- and intra-specific relationships of Paramecium spp.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0077044