Accelerated decline in cardiac stem cell efficiency in Spontaneously hypertensive rat compared to normotensive Wistar rat

• Published in: PloS one 2017-12, Vol.12 (12), p.e0189129-e0189129
• Main Authors: Saheera, Sherin, Nair, Renuka R
• Format: Article
• Language: English
• Subjects: Age; Aging; Aging (natural); Biology and Life Sciences; c-Kit protein; Cardiology; Cell division; Comparative analysis; Coronary artery disease; Deoxyribonucleic acid; Disease control; DNA; DNA damage; Efficiency; Explants; Health aspects; Heart; Heart diseases; Heart failure; Homeostasis; Hypertension; Hypertrophy; Laboratory animals; Maintenance; Medicine and Health Sciences; Myocardium; Oxidative stress; Physical Sciences; Physiology; Rats; Reactive oxygen species; Regeneration; Research and Analysis Methods; Residential density; Risk factors; Risk management; Rodents; Senescence; Stem cell transplantation; Stem cells; Telomerase; Therapeutic applications; Tissue engineering
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0189129

Cardiac hypertrophy is recognized as an independent risk factor for cardiac failure. Efficient management of hypertensive heart disease requires identification of factors that can possibly mediate the transition from hypertrophy to failure. Resident cardiac stem cells have a prominent role in the maintenance of cardiac tissue homeostasis. Decline in the proportion of healthy cardiac stem cells (CSCs) can affect tissue regeneration. In pathological conditions, apart from natural aging, an adverse microenvironment can lead to decrease in efficiency of CSCs. A systematic analysis of cardiac stem cell characteristics in pathological conditions has not been reported so far. Therefore, this study was designed with the objective of examining the age associated variation in stem cell attributes of Spontaneously hypertensive rat (SHR) in comparison with normotensive Wistar rat. Spontaneously hypertensive rat was used as the experimental model since the cardiac remodeling resembles the clinical course of hypertensive heart disease. CSCs were isolated from atrial explants. Stem cell attributes were assessed in 1-week, 6, 12 and 18-month-old male SHR, in comparison with age matched Wistar rats. In 1-week-old pups, stem cell attributes of SHR and Wistar were comparable. Migration potential, proliferative capacity, TERT expression, telomerase activity and the proportion of c-kit+ cells decreased with age, both in SHR and Wistar. DNA damage and the proportion of senescent CSCs increased with age both in SHR and Wistar rats. Age associated increase was observed in the oxidative stress of stem cells, possibly mediated by the enhanced oxidative stress in the microenvironment. The changes were more pronounced in SHR, and as early as six months of age, there was significant decrease in efficiency of CSCs of SHR compared to Wistar. The density of healthy CSCs determined as a fraction of the differentiated cells was remarkably low in 18-month-old SHR. Age associated decrease in functionally efficient CSCs was therefore accelerated in SHR. Considering the vital role of CSCs in the maintenance of a healthy myocardium, decrease in functionally efficient CSCs can be a precipitating factor in pathological cardiac remodeling. Elevated ROS levels in CSCs of SHR lends scope for speculation that decrease in efficiency of CSCs is mediated by oxidative stress; and that modulation of the microenvironment by therapeutic interventions can restore a healthy stem cell population and facilitate