Transcriptomic analysis of chicken Myozenin 3 regulation reveals its potential role in cell proliferation

• Published in: PloS one 2017-12, Vol.12 (12), p.e0189476-e0189476
• Main Authors: Ye, Maosen, Ye, Fei, He, Liutao, Luo, Bin, Yang, Fuling, Cui, Can, Zhao, Xiaoling, Yin, Huadong, Li, Diyan, Xu, Hengyong, Wang, Yan, Zhu, Qing
• Format: Article
• Language: English
• Subjects: Biological activity; Biology and life sciences; Biosynthesis; Cardiomyocytes; Cell cycle; Cell growth; Cell proliferation; Cellular signal transduction; Chickens; Cholecystokinin; Cooperation; Differentiation; Embryo fibroblasts; Embryogenesis; Embryonic development; Enrichment; Fibroblasts; Gene expression; Genes; Genetic aspects; Genetic regulation; Laboratories; Medicine and Health Sciences; Metabolism; Muscles; Musculoskeletal system; Myoblasts; Peroxisome proliferator-activated receptors; Proteins; Ribonucleic acid; RNA; RNA sequencing; Signal transduction; Smooth muscle
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0189476

Embryonic muscle development and fibre type differentiation has always been a topic of great importance due to its impact on both human health and farm animal financial values. Myozenin3 (Myoz3) is an important candidate gene that may regulate these processes. In the current study, we knocked down and overexpressed Myoz3 in chicken embryonic fibroblasts (CEFs) and chicken myoblasts, then utilized RNA-seq technology to screen genes, pathways and biological processes associated with Myoz3. Multiple differentially expressed genes were identified, including MYH10, MYLK2, NFAM1, MYL4, MYL9, PDZLIM1; those can in turn regulate each other and influence the development of muscle fibres. Gene ontology (GO) terms including some involved in positive regulation of cell proliferation were enriched. We further validated our results by testing the activity of cells by cell counting kit-8(CCK-8) and confirmed that under the condition of Myoz3 overexpression, the proliferation rate of CEFs and myoblasts was significantly upregulated, in addition, expression level of fast muscle specific gene was also significantly upregulated in myoblasts. Pathway enrichment analysis revealed that the PPAR (Peroxisome Proliferator-Activated Receptor) pathway was enriched, suggesting the possibility that Myoz3 regulates muscle fibre development and differentiation through the PPAR pathway. Our results provide valuable evidence regarding the regulatory functions of Myoz3 in embryonic cells by screening multiple candidate genes, biological processes and pathways associated with Myoz3.