Whole genomic sequence and replication kinetics of a new enterovirus C96 isolated from Guangdong, China with a different cell tropism

Enterovirus 96 (EV-C96) is a newly described serotype within the enterovirus C (EV-C) species, and its biological and pathological characters are largely unknown. In this study, we sequenced the whole genome of a novel EV-C96 strain that was isolated in 2011 from a patient with acute flaccid paralys...

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Bibliographic Details
Published in:PloS one 2014-01, Vol.9 (1), p.e86877-e86877
Main Authors: Lu, Jing, Zheng, Huanying, Zhang, Yong, Guo, Xue, Wu, De, Li, Hui, Liu, Leng, Zeng, Hanri, Yi, Lina, Fang, Ling, Mo, Yanling, Xu, Wenbo, Ke, Changwen
Format: Article
Language:English
Subjects:
Analysis
Animals
Biodiversity
Biology
Cell Line, Tumor
Cercopithecus aethiops
Child, Preschool
China
Disease control
Disease prevention
Encephalitis
Enterovirus
Enterovirus C, Human - genetics
Enterovirus C, Human - isolation & purification
Enterovirus C, Human - physiology
Enterovirus Infections - virology
Evolution
Female
Genome, Viral
Genomes
Genomics
Health aspects
Humans
Infection
Kinetics
Laboratories
Mutation
Paralysis
Paraplegia - virology
Phylogenetics
Phylogeny
Poliomyelitis
Public health
Recombination
Recombination, Genetic
Replication
Sequence Analysis, DNA
Strains (organisms)
Tropism
Vero Cells
Viral Structural Proteins - genetics
Virology
Virus Replication
Viruses
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Summary:Enterovirus 96 (EV-C96) is a newly described serotype within the enterovirus C (EV-C) species, and its biological and pathological characters are largely unknown. In this study, we sequenced the whole genome of a novel EV-C96 strain that was isolated in 2011 from a patient with acute flaccid paralysis (AFP) in Guangdong province, China and characterized the properties of its infection. Sequence analysis revealed the close relationship between the EV-C96 strains isolated from the Guangdong and Shandong provinces of China, and suggested that recombination events occurred both between these EV-C96 strains and with other EV-C viruses. Moreover, the virus replication kinetics showed EV-C96 Guangdong strain replicated at a high rate in RD cells and presented a different cell tropism to other strains isolated from Shandong recently. These findings gave further insight into the evolutionary processes and extensive biodiversity of EV-C96.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0086877