Elevated MiR-222-3p promotes proliferation and invasion of endometrial carcinoma via targeting ERα

MicroRNAs play key roles in tumor proliferation and invasion. Here we show distinct expression of miR-222-3p between ERα-positive and ERα-negative endometrial carcinoma (EC) cell lines and primary tumors, and investigation of its relationship with ERα and other clinical parameters. In vitro, the fun...

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Bibliographic Details
Published in:PloS one 2014, Vol.9 (1), p.e87563-e87563
Main Authors: Liu, Binya, Che, Qi, Qiu, Haifeng, Bao, Wei, Chen, Xiaoyue, Lu, Wen, Li, Bilan, Wan, Xiaoping
Format: Article
Language:English
Subjects:
Adult
Animals
Antiestrogens
Apoptosis
Biology
Biotechnology
Breast cancer
Cancer therapies
Carcinoma
Cell cycle
Cell Line, Tumor
Cell proliferation
Childrens health
Endometrial cancer
Endometrial Neoplasms - metabolism
Endometrial Neoplasms - pathology
Endometrium
Epigenetics
Estrogen Receptor alpha - biosynthesis
Estrogen Receptor alpha - genetics
Estrogen receptors
Estrogens
Female
G1 Phase
Gene Expression Regulation, Neoplastic - genetics
Genes
Gynecology
Histology
Hospitals
Humans
Invasiveness
Laboratory animals
Lung cancer
Medicine
Metastasis
Mice
MicroRNAs
MicroRNAs - biosynthesis
MicroRNAs - genetics
Middle Aged
miRNA
Neoplasm Invasiveness
Neoplasm Proteins - biosynthesis
Neoplasm Proteins - genetics
Obstetrics
Penicillin
R&D
Raloxifene
Research & development
RNA, Neoplasm - biosynthesis
RNA, Neoplasm - genetics
S Phase
Tumor cell lines
Tumors
Uterine cancer
Xenografts
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Summary:MicroRNAs play key roles in tumor proliferation and invasion. Here we show distinct expression of miR-222-3p between ERα-positive and ERα-negative endometrial carcinoma (EC) cell lines and primary tumors, and investigation of its relationship with ERα and other clinical parameters. In vitro, the function of miR-222-3p was examined in RL95-2 and AN3CA cell lines. MiR-222-3p expression was negatively correlated with ERα. Over-expressed miR-222-3p in RL95-2 cells promoted cell proliferation, enhanced invasiveness and induced a G1 to S phase shift in cell cycle. Furthermore, the miR-222-3p inhibitor decreased the activity of AN3CA cells to proliferate and invade. In vivo, down-regulated miR-222-3p of AN3CA cells inhibited EC tumor growth in a mouse xenograft model. Additionally, miR-222-3p increased raloxifene resistance through suppressing ERα expression in EC cells. In conclusion, miR-222-3p plays a significant role in the regulation of ERα expression and could be potential targets for restoring ERα expression and responding to antiestrogen therapy in a subset of ECs.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0087563