Characterization of a not so new potexvirus from babaco (Vasconcellea x heilbornii)

A new member of the genus Potexvirus was fully sequenced and characterized. The virus was isolated from babaco (Vasconcellea x heilbornii), a natural hybrid native to Ecuador. The virus contains a 6,692 nt long genome organized in five open reading frames in an arrangement typical of other potexviru...

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Bibliographic Details
Published in:PloS one 2017-12, Vol.12 (12), p.e0189519-e0189519
Main Authors: Alvarez-Quinto, Robert A, Cornejo-Franco, Juan F, Quito-Avila, Diego F
Format: Article
Language:English
Subjects:
3' Untranslated regions
Amaranthus dubius
Amino acids
Analysis
Biology and Life Sciences
Carica papaya
Chenopodium amaranticolor
Chenopodium quinoa
Cloning
Coat protein
Computer and Information Sciences
Cucurbita ecuadoriensis
Genes, Viral
Genetic aspects
Genomes
Host range
Host Specificity
Identification and classification
Leaves
Luffa cylindrica
Luffa operculata
Magnoliopsida - virology
Maximum likelihood method
Nicotiana benthamiana
Nucleotide sequence
Open reading frames
Phylogenetics
Phylogeny
Plant viruses
Potexvirus - genetics
Potexvirus - isolation & purification
Potexviruses
Research and Analysis Methods
Senna alata
Senna occidentalis
Sequence Analysis, DNA
Solanum betaceum
Taxonomy
Vasconcellea pubescens
Viral Proteins - genetics
Viral Tropism
Virology
Viruses
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Description
Summary:A new member of the genus Potexvirus was fully sequenced and characterized. The virus was isolated from babaco (Vasconcellea x heilbornii), a natural hybrid native to Ecuador. The virus contains a 6,692 nt long genome organized in five open reading frames in an arrangement typical of other potexviruses. Sequence comparisons revealed close relatedness with Papaya mosaic virus (PapMV), Alternathera mosaic virus (AltMV) and Senna mosaic virus (SenMV), exhibiting nucleotide identities up to 67% for the polymerase (Pol) and 68% for the coat protein (CP), with deduced amino acid identities of 70% and 72% for the Pol and CP, respectively. The presence of an AlkB domain, in the polymerase region, was observed. Terminal nucleotide sequences were conserved across potexviruses with characteristic motifs and predicted secondary structures at the 3' UTR. Although serologically undistinguishable from PapMV and AltMV, the new virus showed differences in host range and symptom induction. The name babaco mosaic virus is proposed for this newly characterized Potexvirus. The complete genome sequence of the new virus has been deposited in NCBI GenBank under accession number MF978248.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0189519