Comparative study of qualitative and quantitative methods to determine toxicity level of Aspergillus flavus isolates in maize

An attempt was made to compare between easy and inexpensive qualitative method (ammonia vapour test) and analytical methods (thin layer chromatography and enzyme-linked immunosorbent assay) for identification of aflatoxigenic isolates of Aspergillus flavus in maize. In this comparative study the tox...

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Published in:PloS one 2017-12, Vol.12 (12), p.e0189760
Main Authors: Shekhar, Meena, Singh, Nirupma, Dutta, Ram, Kumar, Shrvan, Mahajan, Vinay
Format: Article
Language:English
Subjects:
Aflatoxin B1
Agricultural commodities
Ammonia
Analytical methods
Aspergillus
Aspergillus flavus
Biology and Life Sciences
Causes of
Chromatography
Color
Comparative studies
Corn
Cream
Enzyme-linked immunosorbent assay
Food contamination & poisoning
Genetic aspects
Grain
Hepatitis
Identification methods
Medicine and Health Sciences
Methods
Oryza
Physical Sciences
Physiological aspects
Qualitative analysis
Research and Analysis Methods
Test procedures
Thin layer chromatography
Toxicity
Vapors
Virulence (Microbiology)
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description An attempt was made to compare between easy and inexpensive qualitative method (ammonia vapour test) and analytical methods (thin layer chromatography and enzyme-linked immunosorbent assay) for identification of aflatoxigenic isolates of Aspergillus flavus in maize. In this comparative study the toxicity level of A. flavus isolates exhibited 100% agreement among ammonia vapour test, ELISA and TLC for highly toxigenic (>2000 ppb) and toxigenic (501-2000 ppb) isolates while 88.5% agreement observed for least toxic (
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In this comparative study the toxicity level of A. flavus isolates exhibited 100% agreement among ammonia vapour test, ELISA and TLC for highly toxigenic (&gt;2000 ppb) and toxigenic (501-2000 ppb) isolates while 88.5% agreement observed for least toxic (&lt;20 ppb) isolates. In ammonia vapour test 51% of A. flavus isolates showed creamish or no colour change corresponding to least toxic/atoxic (&lt;20ppb) category estimated by ELISA. Similarly 22% highly toxic isolates exhibited plum red colour, 12% moderately toxic indicated pink colour and 10% toxic isolates showed red colour. However, 11.5% isolates were found to be false positive in cream colour category (least toxic) and 28.5% false negatives in pink colour (moderately toxic) category. The isolates from different agroclimatic zones of maize in India showed high variability for aflatoxin B1 (AFB1) production potential ranging from 0.214-8116.61 ppb. Toxigenic potential of Aspergillus flavus isolates in culture was further validated by inoculating maize grain sample with four different isolates with varied toxin producing ability. 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subjects Aflatoxin B1
Agricultural commodities
Ammonia
Analytical methods
Aspergillus
Aspergillus flavus
Biology and Life Sciences
Causes of
Chromatography
Color
Comparative studies
Corn
Cream
Enzyme-linked immunosorbent assay
Food contamination & poisoning
Genetic aspects
Grain
Hepatitis
Identification methods
Medicine and Health Sciences
Methods
Oryza
Physical Sciences
Physiological aspects
Qualitative analysis
Research and Analysis Methods
Test procedures
Thin layer chromatography
Toxicity
Vapors
Virulence (Microbiology)
title Comparative study of qualitative and quantitative methods to determine toxicity level of Aspergillus flavus isolates in maize
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