The interaction effects of pri-let-7a-1 rs10739971 with PGC and ERCC6 gene polymorphisms in gastric cancer and atrophic gastritis

• Published in: PloS one 2014-02, Vol.9 (2), p.e89203-e89203
• Main Authors: Xu, Qian, Liu, Jing-wei, He, Cai-yun, Sun, Li-ping, Gong, Yue-hua, Jing, Jing-Jing, Xing, Cheng-zhong, Yuan, Yuan
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Analysis; Biology; Biomarkers; Breast cancer; Cancer; Cancer genetics; Cancer research; Deoxyribonucleic acid; Development and progression; Disease prevention; DNA; DNA Helicases - genetics; DNA repair; DNA Repair Enzymes - genetics; Education; Female; Gastric cancer; Gastritis; Gastritis, Atrophic - genetics; Gene expression; Genes; Genetic aspects; Genetic polymorphisms; Genetic Predisposition to Disease - genetics; Genotype; Health aspects; Health risks; Helicobacter pylori; Hospitals; Humans; Laboratories; Male; Medicine; MicroRNA; MicroRNAs; MicroRNAs - genetics; Middle Aged; miRNA; Patients; Pepsinogen C - genetics; Poly-ADP-Ribose Binding Proteins; Polymorphism; Polymorphism, Genetic - genetics; Population; Precancerous Conditions - genetics; Predictions; Proteins; Risk; Stomach cancer; Stomach Neoplasms - genetics; Studies; Surgery; Young Adult
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0089203

The aim of this study was to investigate the interaction effects of pri-let-7a-1 rs10739971 with pepsinogen C (PGC) and excision repair cross complementing group 6 (ERCC6) gene polymorphisms and its association with the risks of gastric cancer and atrophic gastritis. We hoped to identify miRNA polymorphism or a combination of several polymorphisms that could serve as biomarkers for predicting the risk of gastric cancer and its precancerous diseases. Sequenom MassARRAY platform method was used to detect polymorphisms of pri-let-7a-1 rs10739971 G → A, PGC rs4711690 C → G, PGC rs6458238 G → A, PGC rs9471643 G → C, and ERCC6 rs1917799 in 471 gastric cancer patients, 645 atrophic gastritis patients and 717 controls. An interaction effect of pri-let-7a-1 rs10739971 polymorphism with ERCC6 rs1917799 polymorphism was observed for the risk of gastric cancer (P interaction = 0.026); and interaction effects of pri-let-7a-1 rs10739971 polymorphism with PGC rs6458238 polymorphism (P interaction = 0.012) and PGC rs9471643 polymorphism (P interaction = 0.039) were observed for the risk of atrophic gastritis. The combination of pri-let-7a-1 rs10739971 polymorphism and ERCC6 and PGC polymorphisms could provide a greater prediction potential than a single polymorphism on its own. Large-scale studies and molecular mechanism research are needed to confirm our findings.