Two pear glutathione S-transferases genes are regulated during fruit development and involved in response to salicylic acid, auxin, and glucose signaling

• Published in: PloS one 2014-02, Vol.9 (2), p.e89926
• Main Authors: Shi, Hai-Yan, Li, Zheng-Hong, Zhang, Yu-Xing, Chen, Liang, Xiang, Di-Ying, Zhang, Yu-Feng
• Format: Article
• Language: English
• Subjects: Acids; Agriculture; Amino Acid Sequence; Amino acids; Arabidopsis thaliana; Barley; Base Sequence; Biology; Cladistic analysis; Cloning; Cluster Analysis; Deoxyribonucleic acid; DNA; DNA Primers - genetics; Fruit - growth & development; Fruit - metabolism; Fruits; Gene expression; Gene Expression Regulation, Plant - drug effects; Gene Expression Regulation, Plant - physiology; Gene Library; Genes; Genomics; Glucose; Glucose - pharmacology; Glutathione; Glutathione transferase; Glutathione Transferase - classification; Glutathione Transferase - genetics; Glutathione Transferase - metabolism; Harvest; Homology; Horticulture; Indoleacetic acid; Indoleacetic Acids - pharmacology; Introns; Library collections; Medical treatment; Metabolism; Molecular Sequence Data; Oxidative stress; Phenols (Class of compounds); Phylogeny; Protein Structure, Tertiary - genetics; Proteins; Proteomics; Pyrus - enzymology; Pyrus pyrifolia; Real-Time Polymerase Chain Reaction; Salicylic acid; Salicylic Acid - pharmacology; Senescence; Sequence Alignment; Sequence Analysis, DNA; Signal Transduction - physiology; Signaling; Splice junctions; Sugar; Thiols
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0089926

Two genes encoding putative glutathione S-transferase proteins were isolated from pear (Pyrus pyrifolia) and designated PpGST1 and PpGST2. The deduced PpGST1 and PpGST2 proteins contain conserved Glutathione S-transferase N-terminal domain (GST_N) and Glutathione S-transferase, C-terminal domain (GST_C). Using PCR amplification technique, the genomic clones corresponding to PpGST1 and PpGST2 were isolated and shown to contain two introns and a singal intron respectively with typical GT/AG boundaries defining the splice junctions. Phylogenetic analysis clearly demonstrated that PpGST1 belonged to Phi class of GST superfamilies and had high homology with apple MdGST, while PpGST2 was classified into the Tau class of GST superfamilies. The expression of PpGST1 and PpGST2 genes was developmentally regulated in fruit. Further study demonstrated that PpGST1 and PpGST2 expression was remarkably induced by glucose, salicylic acid (SA) and indole-3-aceticacid (IAA) treatments in pear fruit, and in diseased fruit. These data suggested that PpGST1 and PpGST2 might be involved in response to sugar, SA, and IAA signaling during fruit development of pear.