High whey protein intake delayed the loss of lean body mass in healthy old rats, whereas protein type and polyphenol/antioxidant supplementation had no effects

• Published in: PloS one 2014-09, Vol.9 (9), p.e109098-e109098
• Main Authors: Mosoni, Laurent, Gatineau, Eva, Gatellier, Philippe, Migné, Carole, Savary-Auzeloux, Isabelle, Rémond, Didier, Rocher, Emilie, Dardevet, Dominique
• Format: Article
• Language: English
• Subjects: Absorptivity; Age; Aging - drug effects; Aging - metabolism; alpha-Macroglobulins - metabolism; Amino acids; Animals; Antioxidants; Antioxidants (Nutrients); Biology and Life Sciences; Body composition; Body mass; Body Weight - drug effects; Carbonyl compounds; Carbonyls; Casein; Chamomile - chemistry; Diet - methods; Dietary Supplements; Dual energy X-ray absorptiometry; Eating - drug effects; Fibrin; Fibrinogen; Fibrinogen - metabolism; Food and Nutrition; Food intake; Glutathione; Glutathione - metabolism; Lean body mass; Leucine; Life Sciences; Liver; Male; Medicine and Health Sciences; Muscle Proteins - agonists; Muscle Proteins - metabolism; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Muscles; Nutrition; Older people; Organ Size - drug effects; Oxidative stress; Physiological aspects; Plant Extracts - administration & dosage; Polyphenols; Protein biosynthesis; Protein synthesis; Proteins; Proteolysis; Rats; Rats, Wistar; Rodents; Sarcopenia; Sarcopenia - prevention & control; Superoxide Dismutase - metabolism; Thiobarbituric Acid Reactive Substances - metabolism; Tocopherol; Vitamin D; Vitamin D - administration & dosage; Vitamin E; Vitamin E - administration & dosage; Whey; Whey protein; Whey Proteins - administration & dosage; α2-Macroglobulin
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0109098

Our aim was to compare and combine 3 nutritional strategies to slow down the age-related loss of muscle mass in healthy old rats: 1) increase protein intake, which is likely to stimulate muscle protein anabolism; 2) use leucine rich, rapidly digested whey proteins as protein source (whey proteins are recognized as the most effective proteins to stimulate muscle protein anabolism). 3) Supplement animals with a mixture of chamomile extract, vitamin E, vitamin D (reducing inflammation and oxidative stress is also effective to improve muscle anabolism). Such comparisons and combinations were never tested before. Nutritional groups were: casein 12% protein, whey 12% protein, whey 18% protein and each of these groups were supplemented or not with polyphenols/antioxidants. During 6 months, we followed changes of weight, food intake, inflammation (plasma fibrinogen and alpha-2-macroglobulin) and body composition (DXA). After 6 months, we measured muscle mass, in vivo and ex-vivo fed and post-absorptive muscle protein synthesis, ex-vivo muscle proteolysis, and oxidative stress parameters (liver and muscle glutathione, SOD and total antioxidant activities, muscle carbonyls and TBARS). We showed that although micronutrient supplementation reduced inflammation and oxidative stress, the only factor that significantly reduced the loss of lean body mass was the increase in whey protein intake, with no detectable effect on muscle protein synthesis, and a tendency to reduce muscle proteolysis. We conclude that in healthy rats, increasing protein intake is an effective way to delay sarcopenia.