Transcriptomic profiling of gametogenesis in triploid Pacific Oysters Crassostrea gigas: towards an understanding of partial sterility associated with triploidy

Triploidy can occur in many animal species but is often lethal. Among invertebrates, amphibians and fishes, triploids are viable although often sterile or infertile. Most triploids of the Pacific oyster Crassostrea gigas are almost sterile (named "3nβ") yet a low but significant proportion...

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Published in:PloS one 2014-11, Vol.9 (11), p.e112094-e112094
Main Authors: Dheilly, Nolwenn M, Jouaux, Aude, Boudry, Pierre, Favrel, Pascal, Lelong, Christophe
Format: Article
Language:English
Subjects:
Amphibia
Amphibians
Animal species
Animals
Apoptosis
Biodiversity and Ecology
Biology
Biology and Life Sciences
Cell cycle
Cell division
Crassostrea - cytology
Crassostrea - genetics
Crassostrea - physiology
Crassostrea gigas
Deoxyribonucleic acid
Differentiation
DNA
DNA microarrays
DNA repair
Environmental Sciences
Female
Females
Gametogenesis
Gene expression
Gene Expression Profiling - methods
Gene Expression Regulation
Genes
Gonads
Infertility, Male
Invertebrates
Life Sciences
Male
Males
Medical research
Meiosis
Mitosis
Ocean, Atmosphere
Oligonucleotide Array Sequence Analysis - methods
Oysters
Pachytene
Repair
Sciences of the Universe
Sex
Sex determination
Sex Differentiation
Sterility
Studies
Triploidy
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cited_by cdi_FETCH-LOGICAL-c726t-e7aa563304709b3ef48b6529361c5ae1042a517ebbe0ae3bff62b5c363ac61963
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creator Dheilly, Nolwenn M
Jouaux, Aude
Boudry, Pierre
Favrel, Pascal
Lelong, Christophe
description Triploidy can occur in many animal species but is often lethal. Among invertebrates, amphibians and fishes, triploids are viable although often sterile or infertile. Most triploids of the Pacific oyster Crassostrea gigas are almost sterile (named "3nβ") yet a low but significant proportion show an advanced gametogenesis (named "3nα"). These oysters thus constitute an interesting model to study the effect of triploidy on germ cell development. We used microarrays to compare the gonad transcriptomes of diploid 2n and the abovementioned triploid 3nβ and 3nα male and female oysters throughout gametogenesis. All triploids displayed an upregulation of genes related to DNA repair and apoptosis and a downregulation of genes associated with cell division. The comparison of 3nα and 3nβ transcriptomes with 2n revealed the likely involvement of a cell cycle checkpoint during mitosis in the successful but delayed development of gonads in 3nα individuals. In contrast, a disruption of sex differentiation mechanisms may explain the sterility of 3nβ individuals with 3nβ females expressing male-specific genes and 3nβ males expressing female-specific genes. The disruption of sex differentiation and mitosis may be responsible for the impaired gametogenesis of triploid Pacific oysters. The function of the numerous candidate genes identified in our study should now be studied in detail in order to elucidate their role in sex determination, mitosis/meiosis control, pachytene cell cycle checkpoint, and the control of DNA repair/apoptosis.
doi_str_mv 10.1371/journal.pone.0112094
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In contrast, a disruption of sex differentiation mechanisms may explain the sterility of 3nβ individuals with 3nβ females expressing male-specific genes and 3nβ males expressing female-specific genes. The disruption of sex differentiation and mitosis may be responsible for the impaired gametogenesis of triploid Pacific oysters. The function of the numerous candidate genes identified in our study should now be studied in detail in order to elucidate their role in sex determination, mitosis/meiosis control, pachytene cell cycle checkpoint, and the control of DNA repair/apoptosis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25375782</pmid><doi>10.1371/journal.pone.0112094</doi><orcidid>https://orcid.org/0000-0002-3675-5013</orcidid><orcidid>https://orcid.org/0000-0002-5150-2276</orcidid><oa>free_for_read</oa></addata></record>
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subjects Amphibia
Amphibians
Animal species
Animals
Apoptosis
Biodiversity and Ecology
Biology
Biology and Life Sciences
Cell cycle
Cell division
Crassostrea - cytology
Crassostrea - genetics
Crassostrea - physiology
Crassostrea gigas
Deoxyribonucleic acid
Differentiation
DNA
DNA microarrays
DNA repair
Environmental Sciences
Female
Females
Gametogenesis
Gene expression
Gene Expression Profiling - methods
Gene Expression Regulation
Genes
Gonads
Infertility, Male
Invertebrates
Life Sciences
Male
Males
Medical research
Meiosis
Mitosis
Ocean, Atmosphere
Oligonucleotide Array Sequence Analysis - methods
Oysters
Pachytene
Repair
Sciences of the Universe
Sex
Sex determination
Sex Differentiation
Sterility
Studies
Triploidy
title Transcriptomic profiling of gametogenesis in triploid Pacific Oysters Crassostrea gigas: towards an understanding of partial sterility associated with triploidy
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