Lipid vesicle shape analysis from populations using light video microscopy and computer vision

We present a method for giant lipid vesicle shape analysis that combines manually guided large-scale video microscopy and computer vision algorithms to enable analyzing vesicle populations. The method retains the benefits of light microscopy and enables non-destructive analysis of vesicles from susp...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2014-11, Vol.9 (11), p.e113405-e113405
Main Authors: Zupanc, Jernej, Drašler, Barbara, Boljte, Sabina, Kralj-Iglič, Veronika, Iglič, Aleš, Erdogmus, Deniz, Drobne, Damjana
Format: Article
Language:English
Subjects:
Algorithms
Automation
Biology and Life Sciences
Cholesterol - chemistry
Comparative analysis
Computer vision
Flow cytometry
Image analysis
Image processing
Image Processing, Computer-Assisted - statistics & numerical data
Light
Light microscopy
Lipids
Membranes
Microscopy
Microscopy, Video - instrumentation
Microscopy, Video - methods
Morphology
Nondestructive testing
Normal Distribution
Particle Size
Phase transitions
Phosphatidylcholines - chemistry
Physics
Population
Populations
Quotients
Research and Analysis Methods
Roundness
Shape
Studies
Suspensions
Unilamellar Liposomes - chemistry
Vesicles
Vision
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We present a method for giant lipid vesicle shape analysis that combines manually guided large-scale video microscopy and computer vision algorithms to enable analyzing vesicle populations. The method retains the benefits of light microscopy and enables non-destructive analysis of vesicles from suspensions containing up to several thousands of lipid vesicles (1-50 µm in diameter). For each sample, image analysis was employed to extract data on vesicle quantity and size distributions of their projected diameters and isoperimetric quotients (measure of contour roundness). This process enables a comparison of samples from the same population over time, or the comparison of a treated population to a control. Although vesicles in suspensions are heterogeneous in sizes and shapes and have distinctively non-homogeneous distribution throughout the suspension, this method allows for the capture and analysis of repeatable vesicle samples that are representative of the population inspected.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0113405