Human adipose derived stem cells regress fibrosis in a chronic renal fibrotic model induced by adenine

ADSCs transplantation had been shown in some experimental models of kidney damage that it improves kidney function and reduces fibrosis. In this study we evaluated the effect of human adipose tissue-derived stem cell (hADSC) therapy in a chronic kidney damage experimental model. A chronic kidney inj...

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Bibliographic Details
Published in:PloS one 2017-12, Vol.12 (12), p.e0187907
Main Authors: Rivera-Valdés, Juan José, García-Bañuelos, Jesus, Salazar-Montes, Adriana, García-Benavides, Leonel, Dominguez-Rosales, Alfredo, Armendáriz-Borunda, Juan, Sandoval-Rodríguez, Ana
Format: Article
Language:English
Subjects:
Adenine
Adenine - toxicity
Adipose tissue
Adipose Tissue - cytology
Analysis
Animals
Biology and Life Sciences
Cells, Cultured
Chemokines
Collagen (type I)
Connective tissue growth factor
Creatinine
Cytokines
Development and progression
Diabetes
Fibrosis
Fibrosis - genetics
Gene Expression Profiling
Gene therapy
Genetic aspects
Genomics
Health sciences
Humans
Interleukin 1
Interleukin 10
Interleukin 6
Kidney Diseases - chemically induced
Kidney Diseases - genetics
Kidney Diseases - prevention & control
Kidneys
Male
Medicine
Medicine and Health Sciences
Molecular biology
mRNA
Physiological aspects
Prevention
Rats
Rats, Wistar
Regression analysis
Renal function
Rodents
Skin & tissue grafts
Stem cell transplantation
Stem cells
Stem Cells - cytology
Therapy
Tissue engineering
Transforming growth factor-b1
Transplantation
Urea
Urine
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Summary:ADSCs transplantation had been shown in some experimental models of kidney damage that it improves kidney function and reduces fibrosis. In this study we evaluated the effect of human adipose tissue-derived stem cell (hADSC) therapy in a chronic kidney damage experimental model. A chronic kidney injury was induced by daily orogastric administration of adenine (100mg/kg) to male Wistar rats for 28 days. hADSCs were isolated, expanded and characterized before transplantation. hADSC administration was performed in a tail vein at a dose of 2 x106 cells/animal. Animals were sacrificed at 7 days post-treatment. The percentage of fibrotic tissue, serum and urine levels of urea, creatinine, total protein and renal mRNA of COL1A1, TGFB1, CTGF, ACTA2, IL6, IL10, TNF were analyzed. hADSCs treatment significantly reduces kidney fibrosis, improves urea and creatinine serum and urine levels, and diminishes COL1A1, TGFB1, CTGF, ACTA2 mRNA kidney levels. These results showed that cell therapy using hADSCs improves renal function and reduces fibrosis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0187907