Lack of K-Dependent Oxidative Stress in Cotton Roots Following Coronatine-Induced ROS Accumulation

• Published in: PloS one 2015-05, Vol.10 (5), p.e0126476-e0126476
• Main Authors: Zhang, Zhiyong, Zhang, Xin, Hu, Zebing, Wang, Sufang, Zhang, Jinbao, Wang, Xiaojing, Wang, Qinglian, Zhang, Baohong
• Format: Article
• Language: English
• Subjects: Accumulation; Amino Acids - pharmacology; Analysis; Antioxidants; Antioxidants (Nutrients); Antioxidants - pharmacology; Arabidopsis thaliana; Collaboration; Coronatine; Cotton; Enzymes; Gene expression; Gossypium - anatomy & histology; Gossypium - drug effects; Gossypium - growth & development; Growth regulators; Health aspects; Hydrogen; Hydrogen peroxide; Hydroponics; Indenes - pharmacology; Jasmonic acid; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Malondialdehyde - metabolism; Oryza; Oxidative stress; Oxidative Stress - drug effects; Oxygen; Peroxidation; Plant growth; Plant Roots - drug effects; Plant Roots - growth & development; Potassium; Potassium - metabolism; Reactive oxygen species; Reactive Oxygen Species - metabolism; Ricinus communis; Rodents; Roots; Scavenging; Science; Seedlings; Senescence; Staining; Stress, Physiological - drug effects; Superoxide
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0126476

Coronatine [COR] is a novel type of plant growth regulator with similarities in structure and property to jasmonate. The objective of this study was to examine the relationship between increased root vitality induced by 10 nM COR and reactive oxygen species scavenging under potassium (K)-replete (2.5 mM) and K-deficient (0.05 mM) conditions in hydroponic cultured cotton seedlings. K-replete and K-deficient conditions increased root vitality by 2.7- and 3.5-fold, respectively. COR treatment significantly decreased lipid peroxidation in cotton seedlings determined by reduction in MDA levels. These results suggest that COR improves the functioning of both enzymatic and non-enzymatic antioxidant systems. Under K-replete and K-deficient conditions, COR significantly increased the activities of antioxidant enzymes SOD (only for K-repletion), CAT, GPX, and APX comparing; COR also significantly increased DPPH-radical scavenging activity. However, COR led to 1.6- and 1.7-fold increases in superoxide anion (O2•-) concentrations, and 5.7- and 2.1-fold increases in hydrogen peroxide (H2O2) levels, respectively. Additionally, COR intensified the DAB staining of H2O2 and the NBT staining of O2•-. Therefore, our results reveal that COR-induced ROS accumulation stimulates the activities of most antioxidant enzymes but does not induce oxidative stress in cotton roots.