The Brassicaceae-specific EWR1 gene provides resistance to vascular wilt pathogens

Soil-borne vascular wilt diseases caused by Verticillium spp. are among the most destructive diseases worldwide in a wide range of plant species. The most effective means of controlling Verticillium wilt diseases is the use of genetic resistance. We have previously reported the identification of fou...

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Published in:PloS one 2014-02, Vol.9 (2), p.e88230-e88230
Main Authors: Yadeta, Koste A, Valkenburg, Dirk-Jan, Hanemian, Mathieu, Marco, Yves, Thomma, Bart P H J
Format: Article
Language:English
Subjects:
Activation
Agriculture
Agrobacterium
antimicrobial peptides
Arabidopsis
Arabidopsis - genetics
Arabidopsis thaliana
asymmetric interlaced pcr
Bacteria
Biology
Brassica
Brassicaceae
Brassicaceae - genetics
Brassicaceae - microbiology
Cloning
dahliae
defense peptides
Deoxyribonucleic acid
Disease
Disease Resistance
Diseases
DNA
Gene expression
Genes, Plant
Genomes
Genomics
Homology
Insertion
Laboratories
Life Sciences
Microbial drug resistance
Mutants
Mutation
Overexpression
Pathogens
Peptides
plant
Plant diseases
Plant Diseases - genetics
Plant Diseases - microbiology
Plants, Genetically Modified - genetics
Potatoes
proteins
tomato ve1
Verticillium - physiology
Verticillium wilt
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Valkenburg, Dirk-Jan
Hanemian, Mathieu
Marco, Yves
Thomma, Bart P H J
description Soil-borne vascular wilt diseases caused by Verticillium spp. are among the most destructive diseases worldwide in a wide range of plant species. The most effective means of controlling Verticillium wilt diseases is the use of genetic resistance. We have previously reported the identification of four activation-tagged Arabidopsis mutants which showed enhanced resistance to Verticillium wilt. Among these, one mutant also showed enhanced resistance to Ralstonia solanacearum, a bacterial vascular wilt pathogen. Cloning of the activation tag revealed an insertion upstream of gene At3g13437, which we designated as EWR1 (for Enhancer of vascular Wilt Resistance 1) that encodes a putatively secreted protein of unknown function. The search for homologs of Arabidopsis EWR1 (AtEWR1) in public databases only identified homologs within the Brassicaceae family. We subsequently cloned the EWR1 homolog from Brassica oleracea (BoEWR1) and show that over-expression in Arabidopsis results in V. dahliae resistance. Moreover, over-expression of AtEWR1 and BoEWR1 in N. benthamiana, a member of the Solanaceae family, results in V. dahliae resistance, suggesting that EWR1 homologs can be used to engineer Verticillium wilt resistance in non-Brassicaceae crops as well.
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M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Brassicaceae-specific EWR1 gene provides resistance to vascular wilt pathogens</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-02-05</date><risdate>2014</risdate><volume>9</volume><issue>2</issue><spage>e88230</spage><epage>e88230</epage><pages>e88230-e88230</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Soil-borne vascular wilt diseases caused by Verticillium spp. are among the most destructive diseases worldwide in a wide range of plant species. The most effective means of controlling Verticillium wilt diseases is the use of genetic resistance. We have previously reported the identification of four activation-tagged Arabidopsis mutants which showed enhanced resistance to Verticillium wilt. Among these, one mutant also showed enhanced resistance to Ralstonia solanacearum, a bacterial vascular wilt pathogen. Cloning of the activation tag revealed an insertion upstream of gene At3g13437, which we designated as EWR1 (for Enhancer of vascular Wilt Resistance 1) that encodes a putatively secreted protein of unknown function. The search for homologs of Arabidopsis EWR1 (AtEWR1) in public databases only identified homologs within the Brassicaceae family. We subsequently cloned the EWR1 homolog from Brassica oleracea (BoEWR1) and show that over-expression in Arabidopsis results in V. dahliae resistance. Moreover, over-expression of AtEWR1 and BoEWR1 in N. benthamiana, a member of the Solanaceae family, results in V. dahliae resistance, suggesting that EWR1 homologs can be used to engineer Verticillium wilt resistance in non-Brassicaceae crops as well.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24505441</pmid><doi>10.1371/journal.pone.0088230</doi><tpages>e88230</tpages><orcidid>https://orcid.org/0000-0003-2012-6500</orcidid><oa>free_for_read</oa></addata></record>
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1932-6203
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source Publicly Available Content Database; PubMed Central
subjects Activation
Agriculture
Agrobacterium
antimicrobial peptides
Arabidopsis
Arabidopsis - genetics
Arabidopsis thaliana
asymmetric interlaced pcr
Bacteria
Biology
Brassica
Brassicaceae
Brassicaceae - genetics
Brassicaceae - microbiology
Cloning
dahliae
defense peptides
Deoxyribonucleic acid
Disease
Disease Resistance
Diseases
DNA
Gene expression
Genes, Plant
Genomes
Genomics
Homology
Insertion
Laboratories
Life Sciences
Microbial drug resistance
Mutants
Mutation
Overexpression
Pathogens
Peptides
plant
Plant diseases
Plant Diseases - genetics
Plant Diseases - microbiology
Plants, Genetically Modified - genetics
Potatoes
proteins
tomato ve1
Verticillium - physiology
Verticillium wilt
title The Brassicaceae-specific EWR1 gene provides resistance to vascular wilt pathogens
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