Does 'you are what you eat' apply to mangrove grapsid crabs?

• Published in: PloS one 2014-02, Vol.9 (2), p.e89074
• Main Authors: Bui, Thi Hong Hanh, Lee, Shing Yip
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Assimilation; Australia; Avicennia - chemistry; Bioindicators; Biology; Biomarkers; Brachyura - physiology; Carbon; Carbon Isotopes - metabolism; Carbon sources; Cellulase; Consumers; Consumption; Crabs; Crustaceans; Data processing; Detritivores; Detritus; Diet; Ecosystem; Ecosystems; Estuaries; Feeding; Feeding Behavior - physiology; Food analysis; Food Chain; Food chains; Food production; Food quality; Food webs; International economic relations; Intestinal Absorption - physiology; Isotopes; Leaf litter; Leaves; Male; Mangroves; Muscles; Nitrogen Isotopes - metabolism; Parasesarma erythodactyla; Physiological aspects; Plant Leaves - chemistry; Seafood industry; Sediments (Geology); Stable isotopes; Studies
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0089074

In tropical mangroves, brachyuran crabs have been observed to consume high percentages of leaf litter production. However, questions concerning their ability to assimilate this low-quality food remain, as stable isotope analysis of C and N does not seem to support assimilation. Individuals of the common eastern Australian mangrove grapsid Parasesarma erythodactyla feeding on a mangrove leaf litter or mangrove+microphytobenthos diet developed a significantly higher hepatosomatic index than those with access to only sediment. Lipid biomarker analysis and feeding experiments using (13)C and (15)N-enriched mangrove leaf litter confirmed rapid assimilation of mangrove C and N by P. erythodactyla. Eight-week feeding experiments utilizing three food types (mangrove leaf litter, microphytobenthos and prawn muscle) established different food-specific trophic discrimination values (Δδ(13)C and Δδ(15)N) that are significantly different from those commonly applied to mixing model calculations. The mean Δδ(13)C(crab-mangrove) of +5.45‰ was close to the mean and median literature values for grapsid-mangrove pairs in 29 past studies (+5.2 ± 1.8‰ and +5.6‰, respectively), suggesting that this large discrimination may generally be characteristic of detritivorous grapsid crabs. Solutions from the IsoConc mixing model using our determined trophic discrimination values suggest significantly higher and dominant contributions of mangrove C to the diet than those based on the global mean trophic discrimination values. Our results reaffirm the physiological capacity for and important mediating role of grapsid crabs in processing low-quality mangrove C in tropical estuaries, and caution against the use of global trophic discrimination values in stable isotope analysis of food-web data, especially those involving detritivores. While recent studies have questioned the trophic significance of mangrove detritus in coastal food chains, the contribution of this productive carbon source needs to be re-assessed in the light of these data.