RNA-Seq analysis implicates detoxification pathways in ovine mycotoxin resistance

• Published in: PloS one 2014-06, Vol.9 (6), p.e99975
• Main Authors: Zhang, Jinbi, Pan, Zengxiang, Moloney, Stephanie, Sheppard, Allan
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; Biology and Life Sciences; Climate change; Cytochrome; Cytochrome P450; Detoxification; Disease Resistance; Economic impact; Enzymes; Exposure; Food; Gene expression; Gene Ontology; Genes; Hepatology; Inactivation, Metabolic; Library collections; Liver - drug effects; Liver - metabolism; Medical research; Medicine and Health Sciences; Metabolism; Metabolites; Mycotoxicosis - genetics; Mycotoxicosis - metabolism; Mycotoxicosis - veterinary; Mycotoxins; Mycotoxins - metabolism; Mycotoxins - toxicity; Oxidation resistance; Oxidative stress; Oxygen; Pathways; Physiology; Poisoning; Potentiation; Reactive oxygen species; Ribonucleic acid; RNA; Secondary metabolites; Sequence Analysis, RNA; Sheep; Sheep Diseases - genetics; Sheep Diseases - metabolism; Sheep, Domestic; Signal transduction; Signaling; Transcriptome; Tumors; Wnt protein; Wnt Signaling Pathway
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0099975

Mycotoxin induced hepatoxocity has been linked to oxidative stress, resulting from either an increase in levels of reactive oxygen species (ROS) above normal levels and/or the suppression of antioxidant protective pathways. However, few detailed molecular studies of mycotoxicoses in animals have been carried out. This study use current RNA-seq based approaches to investigate the effects of mycotoxin exposure in a ruminant model. Having first assembled a de novo reference transcriptome, we use RNA-Seq technology to define in vivo hepatic gene expression changes resulting from mycotoxin exposure in relationship to pathological effect. As expected, characteristic oxidative stress related gene expression is markedly different in animals exhibiting poorer outcomes. However, expression of multiple genes critical for detoxification, particularly members of the cytochrome P450 gene family, was significantly higher in animals exhibiting mycotoxin tolerance ('resistance'). Further, we present novel evidence for the amplification of Wnt signalling pathway activity in 'resistant' animals, resulting from the marked suppression of multiple key Wnt inhibitor genes. Notably, 'resistance' may be determined primarily by the ability of an individual to detoxify secondary metabolites generated by the metabolism of mycotoxins and the potentiation of Wnt signalling may be pivotal to achieving a favourable outcome upon challenge.